SciELO - Scientific Electronic Library Online

vol.42 issue112First record of predation on Rhinella diptycha (Anura, Bufonidae) by Caiman latirostris (Crocodylia, Alligatoridae) author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google


Actualidades Biológicas

Print version ISSN 0304-3584


MADRID, Melissa; RESTREPO, Paula A.  and  TRUJILLO-VARGAS, Claudia M.. Differential expression of LRBA protein in human naive and memory B lymphocytes. Actu Biol [online]. 2020, vol.42, n.112, e5. ISSN 0304-3584.

LRBA deficiency (Lipopolysaccharide-responsive and beige-like anchor protein) is a primary immunodeficiency caused by a defective expression of the intracellular protein LRBA, due to homozygous and heterozygous composed mutations in the corresponding gene. Patients with this immunodeficiency have a wide spectrum of clinical manifestations that include mainly immunodeficiency and autoimmunity. LRBA deficiency is associated with a decrease in class-switched memory B cells in peripheral blood and plasmablasts count, and a specific antibody response defective, as well as a defect in apoptosis and autophagy. In this study, intracellular differential expression of the LRBA protein in naïve and memory B cells of human peripheral blood was evaluated under different conditions by flow cytometry. We evidence that memory B cells have a higher expression of LRBA; on the other hand, we demonstrate that the expression of this protein in the B cells increases considerably under activation conditions; however; the magnitude of the increase in LRBA expression under this condition is higher in naïve B cells. In contrast, we show that the expression of this protein decreases in B cells undergoing to apoptosis, although even under these conditions, it is higher in memory B cells. These findings suggest that LRBA plays an important role in the development of humoral immune memory.

Keywords : Apoptosis; flow cytometry; primary immunodeficiency diseases; naïve B cells; memory B cells; proliferation assays.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )