Abstract

SALAZAR-MOSCOSO, Yuly Marcela; MARTINEZ-GARRO, Juliana; GUZMAN-GONZALEZ, Pablo Andrés  and  PLESE, Tinka. DNA extraction using minimally invasive samples in Xenarthra order Pilosa, a contribution to their conservation. Actu Biol [online]. 2022, vol.44, n.116, e6.  Epub May 26, 2022. ISSN 0304-3584.  https://doi.org/10.17533/udea.acbi.v44n116a06.

Xenarthrans are a group of mammals of great historical and ecological importance originated in South America. The implementation of molecular genetics techniques in these animals are on the rise with the promise of expanding our knowledge. The minimally invasive sampling methods are a success tool for genetic monitoring in conservation and we probe that could be used for future studies in xenarthrans. We compared the quality and quantity of DNA extracted from blood, tissue, saliva, feces, and hair using two commercial extraction kits: PrepFiler™ and GeneJET™. DNA concentration, purity and integrity were determined using Spectrophotometry, and electrophoresis, respectively. A two-factor mixed ANOVA and Tukey Multiple Comparison Test were used to compare mean DNA concentrations between DNA extraction methods and across biological sample types. The highest yields were of DNA obtained from tissue sample with PrepFiler™ kit, with means in amount (5.25 ng/µL) and purity (1.87) higher than the other samples, and clear and integrated bands on the electrophoresis gel. However, we cannot recommend the use of this sample in live animals.

The DNA obtained from saliva with the extraction kit PrepFiler™ offers similar results in terms of amount (mean 3.56 ng/µL), purity (1.85) and integrity of the DNA, and the Tukey comparison shown than between saliva and blood does not exist significant differences (p=0.1028), and the obtainment of saliva samples requires less intervention to the animal. For this reason, we concluded DNA extraction using the PrepFiler™in saliva samples is the best option for extracting high quality DNA in studied species.

Keywords : DNA; Bradypodidae; DNA integrity; Genetic; Myrmecophagidae.

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