Abstract

CORREA, Hebelin et al. RÁPIDA CONSTRUCCIÓN DE UNA LIBRERÍA DE LOS PRODUCTOS DE CULTIVO DE 14 BACTERIAS DEL PHYLUM FIRMICUTES SIMBIONTES DEL OCTOCORAL PSEUDOPTEROGORGIA ELISABETHAE DE LA ISLA DE PROVIDENCIA (CARIBE SUR-OCCIDENTAL). Rev. acad. colomb. cienc. exact. fis. nat. [online]. 2011, vol.35, n.136, pp.337-348. ISSN 0370-3908.

This paper describes a convenient strategy to accelerate and simplify the detection and identification of compounds produced in four different culture media, by 14 bacteria of the phylum Firmicutes, symbionts of the octocoral Pseudopterogorgia elisabethae collected at Providencia Island, South-West Caribbean. The process involved: bacterial culture miniaturization, solid phase extraction of the products using the resin Diaion^TM HP-20, the analysis by UPLC-MS of the products and finally the generation of the library, that allows to discard culture media components detecting 46 compounds produced by all bacteria and the subsequent identification of 10 of them, using the database Antibase 2007^TM as a tool for dereplication of these substances.

Keywords : library of the products produced by bacteria belonging to the phylum Firmicutes; chemical screening of compounds produced by bacteria belonging to the phylum Firmicutes; Pseudopterogorgia elisabethae-associated bacteria; UPLC-MS; Identification by Antibase 2007^TM.

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