Abstract

CALA, Mónica et al. ESTUDIO COMPARATIVO POR ELECTROFORESIS CAPILAR Y CROMATOGRAFÍA LÍQUIDA DE ALTA EFICIENCIA DE CATEQUINAS EXTRAÍDAS DE CINCO VARIEDADES DE CACAO COLOMBIANO. Rev. acad. colomb. cienc. exact. fis. nat. [online]. 2011, vol.35, n.136, pp.371-379. ISSN 0370-3908.

Analytical methodologies based on capillary electrophoresis (CE) and high performance liquid chromatography (HPLC) were implemented for the separation and quantitation of (±)-catechine, (-)-epicatechine, (-)-epigallocatechine, (-)-epicatechine gallate and (-)-epigallocatechine gallate in 5 samples of 3 cacao varieties grown in Colombia. HPLC was found to be more reproducible than CE, which turned out to be faster, more economical and less contaminant. (±)-Catechine (0,07- 0,79 mg /g cacao) and (-)-epicatechine (1,99-9,66 mg /g cacao) were determined in aqueous extracts of the 3 cacao varieties.

Keywords : catechines; CE; HPLC; Theobroma cacao.

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