Resumo

PEREZ-JARAMILLO, Claudia C.; SANCHEZ-PERALTA, Wilmer Fernando; MURILLO-ARANGO, Walter  e  MENDEZ-ARTEAGA, John J.. Joint antioxidant action of ethanolic extracts of Mollinedia lanceolata , Croton leptostachyus and Siparuna sessiliflora. Rev. acad. colomb. cienc. exact. fis. nat. [online]. 2017, vol.41, n.158, pp.64-70. ISSN 0370-3908.  https://doi.org/10.18257/raccefyn.425.

In recent years, oxidative damage has been associated with diseases such as cancer, diabetes and Alzheimer, among others. The consumption of antioxidants, especially from vegetable sources, has been proposed as an alternative for prevention and treatment; however, few studies have been conducted on the effect of mixtures of antioxidant compounds or matrices that include them. In this study we used crude extracts and mixtures of extracts from Mollinedia lanceolata, Siparuna sessiliflora and Croton leptostachyus. We prepared crude ethanolic extracts from the three plants previously characterized with phytochemical procedures. We performed indirect antioxidant tests with the DPPH and ABTS radicals, we determine the various IC50 used as the basis for preparing 1:1 mixtures with all the extracts and we reassess their antioxidant and antiemolitic activity, as well as their combination index using the free-access software CompuSyn. We found polyphenols, tannins, flavonoids, terpenes, steroids and alkaloids. The most active extract in the stabilization of the DPPH radical was C. leptostachyus (IC50 = 53.5 ± 1.1 mg/l) and of ABTS, M. lanceolata (IC50 = 128.8 ± 0.9 mg/ l). All mixtures acted in synergy to stabilize DPPH with combination indices (CI) higher than 1, whereas in ABTS mixtures they were antagonistic (CI>1), except for M. lanceolata + S. sessiliflora (CI<1). None of the extracts presented an antihemolytic effect manifested in higher hemolysis rates, especially in the mixtures. © 2017. Acad. Colomb. Cienc. Ex. Fis. Nat.

Palavras-chave : Antioxidant activity; Plant extracts; Joint action; Synergism; Antagonism; Monimiaceae; Euphorbiaceae; Siparunaceae.

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