Abstract

COY-RODRIGUEZ, Vivian Andrea; VARGAS-OSORIO, Lily Julieth; VARON-LOPEZ, Maryeimy  and  LOPEZ-CARDONA, Nathali. Identification of fungi and bacteria associated with candahar stems ( Gmelina arborea ROXB) in the department of Tolima, Colombia. Rev. acad. colomb. cienc. exact. fis. nat. [online]. 2018, vol.42, n.165, pp.343-352. ISSN 0370-3908.  https://doi.org/10.18257/raccefyn.771.

Gmelina (Gmelina arborea Roxb) is a species of global distribution with a high commercial value due to its rapid growth and excellent physical, chemical, and biological features. Fungi and bacteria are the most important microorganisms that can affect its production. The present study aimed at isolating and identifying fungi and bacteria associated with G. arborea stem diseases in plantations located in the department of Tolima (Colombia). The morphological characterization of the fungal colonies was carried out in Potato Dextrose Agar medium (PDA) with subsequent microscopic identification after inducing the reproductive structures. Bacteria isolation was conducted in nutrient agar with subsequent biochemical characterization. The molecular identification was made by the amplification of the ITS region for fungi and the 16S for bacteria. We identified the fungi Lasiodiplodia theobromae, Coniothyrium aleuritis, Curvularia geniculata, Chaetomium globosum, Phomopsis columnaris, Diaporthe sp, as well as an isolate belonging to the Dothideomycetes class. The bacteria corresponded to Bacillus cereus, Pseudomonas alcaligenes, and Pantoea dispersa. The pathogenicity tests determined that L. theobromae and C. geniculate caused rot of vascular tissue in young candahar stems.

Keywords : Forest species; ITS; 16S; Koch’s postulates; Microorganisms..

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