Abstract

OSORIO, José Henry  and  QUENAN, Yoccner Edilson. MODIFICATION OF A DNA EXTRACTION TECHNIQUE FROM WHITE HUMAN BLOOD CELLS. Biosalud [online]. 2012, vol.11, n.2, pp.20-25. ISSN 1657-9550.

Introduction: The modified techniques for DNA extraction from white blood cells, allow obtaining a good efficiency in the extracted DNA quantity and purity. Objective: To modify the DNA extraction technique in order to save time and necessary reactives. Materials and Methods: Gustafson's /1987) extraction technique steps were modified. The quantity of DNA produced was calculated using spectrophotometry. The DNA purity was assessed through PCR amplification and confirmed using agarose gels. Results: The changes proposed to Gustafson's DNA extraction method, represent 70% savings in money, and a 6 hours savings in time approximately. Conclusion: The method proposed in the present work is more economic in time and money for white blood cells DNA extraction. Also the efficiency as far as quantity of DNA produced is higher.

Keywords : DNA; extraction methods; nucleic acids.

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