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Biotecnología en el Sector Agropecuario y Agroindustrial

versão impressa ISSN 1692-3561

Resumo

HOYOS, JOSÉ LUIS; PEREA ROMAN, CRÍSPULO  e  VELASCO M, REINALDO J. EVALUATION OF THE EFFECT OF DIFFERENT CONCENTRATIONS OF PHYTOHORMONES IN MICROPROPAGATION OF DOMINICO HARTÓN PLANTAIN (Musa AAB Simmonds). Rev.Bio.Agro [online]. 2008, vol.6, n.2, pp.99-104. ISSN 1692-3561.

The effect of the hormonal concentration in the generation of bud in micropropagation of dominico-harton plantain (Musa AAB Simmonds) was evaluated with 10 treatments in a Complete Randomized Design (CRD). Three concentrations of BAP and IAA in a 3x3 factorial arrangement: 0,01; 0,5 and 5 mg/L were tested. The treatments presented significant differences (p< 0.01%) in vitro multiplication phase. The MSDt test showed that the major number of bud were found with 5mg/L of BAP / 0.5mg/L of AIA and 5mg/L of BAP / 0.01mg/L of AIA combinations. To determine the optimum concentration of AIA from the resultant rank (0,01-0,5 mg/L), an aditional experiment with 6 treatments in a Complete Randomized Design (CRD), varying only AIA concentration was conducted. The concentrations 0.2 and 0,3 mg/L of AIA (p< 0.01%) generated the biggest number of sprouts in the stage of multiplication

Palavras-chave : Micropropagation; plantain; hormones; multiplication; buds.

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