Abstract

TORRES RODRIGUEZ, GERARDO ANDRÉS. APPLICATION OF PLASMA COAGULATE METHOD FOR CELULAR ULTRASTRUCTURE STUDIES. Rev.Bio.Agro [online]. 2009, vol.7, n.1, pp.8-14. ISSN 1692-3561.

Studying ultrastructure cell in particle material such as bacteria, virus and cells suspension require special handling. For that reason, it has been developed techniques like plasma coagulate with phagocytosis as experimental model and analyzed by transmission electronic microscopy (TEM) and scanning electronic microscopy (SEM). Four stages of phagocytosis were observed. The efficient proportion to observe the phagocytosis process was 10 particles per NPM (Nuclear Polymorphic), although 20 min as a incubation period was very short to reaching the total process. Applying this technique, cells conserved their integrity to obtain good quality preparation for TEM. In contrast for SEM samples which kept coagulate fiber components adhered to the surface, producing devices and cell deterioration was observed.

Keywords : Electronic microscopy; phagocytosis; plasma coagulate; lymphocite.

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