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Biotecnología en el Sector Agropecuario y Agroindustrial
versão impressa ISSN 1692-3561
Resumo
ACOSTA R., ALEYDA M.; PENA S., ENRIQUE J.; PAEZ, MARTHA I. e BENITEZ B., RICARDO. CORRELATION OF THE CONCENTRATION POLYAMINES WITH THE 'IN VITRO' DEVELOPMENT OF Annona muricata L.. Rev.Bio.Agro [online]. 2012, vol.10, n.2, pp.71-78. ISSN 1692-3561.
Annona muricata is promising for crop because of its importance like food and exquisite fruit. The recalcitrant nature of the vegetative buds of the specie has dificulted the micropropagation of plant material mature that previously it was selected for its high productivity. This study aimed to identify the relationship between plant development 'In Vitro' of Annona muricata and endogenous levels of polyamines. Plant development 'In Vitro' was promoted by serial micrografting and the endogenous levels of polyamines were quantified by High-Performance Liquid Chromatography (HPLC). The results indicate that spermine is the most abundant in shoots (54,5%), followed by putrescine (27,6%) and spermidine (17,9%). The spermine decreases with increasing series of micrografting and age of the shoot, this situation is similar for the total content of polyamines. This research found several correlations between plant development 'in Vitro' of Annona muricata and polyamines, reaffirming their role as physiological markers.
Palavras-chave : Micrografting; Putrescine; Spermidine; Spermine.