Abstract

MONTOYA R., CAROLINA; LOPEZ H., ALBEIRO  and  PARRA S., JAIME. ALTERATIONS IN THE PRODUCTION mRNA OF INTESTINAL ENZYMES IN PIGS DURING SEVERAL POSTWEANING PERIODS. Rev.Bio.Agro [online]. 2012, vol.10, n.2, pp.126-134. ISSN 1692-3561.

The aim of this study was to determine the gene expression of enzymes to enterocyte level of pigs during post-weaning periods.The experiment was conducted in San PabloProductionCenter of the Universidad Nacional de Colombia (Medellín) with 16 weaned pigs at 21 days of age.The animals were fed for 10 days with a basal diet with milk and some of its derivatives, and that also fulfilled all the nutritionals minimums. Pigs were sequentially slaughtered on days one, five, seven, and 10 days after weaning.Complete extraction of small intestine was realized, which was divided into three sections (duodenum, jejunum and ileum). The gene expression of digestive enzymes by RT-PCR was evaluated.The statistical design used was completely at random. For intestinal enzymes significant decreases were showed, where on fifth day post-weaning presented the lowest values (P<0.01). Between one and 10 days post-weaning there were differences (P<0.01). The duodenum showed the highest values of gene expression (P<0.01). Early weaning alters molecular expression of enzymes to enterocyte level, causing the decrease of intestinal absorption of nutrients, and probably the presentation of post-weaning diarrhea syndrome.

Keywords : Aminopeptidases; Disaccharidases; Intestinal villi; RT-PCR; Weaning.

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