Abstract

TORRES, LUZ ANGELA; SUAREZ, ISIDRO ELIAS  and  GATTI, KELLEN. IN VITRO PROPAGATION OF Acacia mangium Willd. Rev.Bio.Agro [online]. 2013, vol.11, n.1, pp.81-87. ISSN 1692-3561.

Acacia (Acacia mangium Willd) is one of the most planted forest species because of the quality of its wood; however, and despite its importance is not easy to find juvenile plant material in adult trees to be used as a source for vegetative propagation. The present research had as objective to develop a micropropagation protocol for acacia from preexisting meristems. The explants consisted of stem section isolated from three months old plants maintained in under greenhouse condition sand shoots from in vitrogerminatedseeds. Surface disinfection was done with different sodium hypochlorite (NaOCl) concentrations plus antibiotics. Disinfected shoots were established in semisolid MS with different BAP concentrations (0; 0,44; 0,88 and 2,22 µM). Multiplied shoots were rooted using different NAA and IBA amounts and finally shoots with, or without, roots were transferred to ex vitro conditions to evaluate field adaptation. The data showed that 1,0% NaOCl and cefalexina (2 mg L^-1) allowed the highest percentage of non-contaminated explants (67%). Explants cultured with 2,22 µM BAP produced the highest number of new shoots, while 2,69 µM ANA induced more roots per shoot. An 87% survival was observe

Keywords : Micropropagation; Benzilaminopurine; Preexisting meristems; Forest tree.

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