Resumo

ARROYAVE R., ANA LORENA; CARDONA B., MARIANA  e  AGUDELO E., LINA MARÍA. IDENTIFICATION OF STRAINS NATIVE WITH POTENTIAL TO OBTAIN POLYHYDROXYALKANOATES - (PHAS) IN ACTIVATED SLUGDES. Rev.Bio.Agro [online]. 2013, vol.11, n.spe, pp.69-76. ISSN 1692-3561.

Polyhydroxyalkanoates are natural polyesters with excellent characteristics to replace conventional plastics. They are bio-compatibles and biodegradables and in a short time they can be reduced to carbon dioxide and water. They are intracellular synthetized by different bacteria and they can be obtained from a large variety of substrates, including some agriculture wastes. The main target is to identify native strains with potential to produce polyhydroxyalkanoates. A bio-prospection was performed in the sludge of San Fernando's waste water plant (Itagüí-Antioquia). 19 colonies were isolated in a selective agar, and then the colonies with positive oxidase were selected. The activation and cultivation were carried in a liquid minimum salt medium (MSM) to establish the biopolymer accumulation rate. The strains LM-3D, LAR-4D, LAR-5B, LAR-5E exhibiteda pHAs accumulation rate of 2, 10, 4, 3% respectively, and the strain LM-3F exhibited a 25% of biopolymer accumulation rateagainst others. Upon the biochemical analysis it was established that polymer producer strains were Shingomonas paucemobilis, Aeromonas hydrophila and Aeromonas sobria.

Palavras-chave : Biopolymers; Bio-prospection; Wasted wáter; Isolation; Activated slugde; Mixed liquor.

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