Abstract

LENIS V., LUÍS ALBERTO; RUALES C., CRISTINA  and  BENITEZ B., RICARDO. DETERMINATION OF GLYCOSIDES, IN EXPRESS HONEY OF STEVIA (Stevia rebaudiana) BY LIQUID CHROMATOGRAPHY. Rev.Bio.Agro [online]. 2013, vol.11, n.spe, pp.85-91. ISSN 1692-3561.

The aim of this study was to incorporate naturally into honey the main sweet diterpene glycosides found in Stevia rebaudiana, called stevioside and rebaudioside A, in order to combine the sweetening properties of these two substances. To determine their degree of incorporation, the diterpene glycosides were identified and quantified both in the prepared syrups and in the honey obtained by the analytical technique of high performance liquid chromatography (HPLC) using a NH2 column (Zorbax-Agilent) and, as a mobile phase, a mixture of acetonitrile: water (70:30) with ultraviolet detection to 194 nm. Quantification was performed by means of a calibration curve where high rates of incorporation, exceeding 97% for stevioside and rebaudioside A, were found. For the standardization of the analytical technique, parameters were determined such as linearity, analytical sensitivity, detection limit, quantification limit and precision, showing that the method developed is simple, fast and reliable within the established limits, and levels up to 0,08 ppm for stevioside and 0,09 ppm for rebaudioside A.

Keywords : Stevioside; Rebaudioside A; HPLC; Quantification.

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