Resumen

PRIETO S, GLORIA ASTRID; PEREA V, YANNETH AIDE  y  ORTIZ L, CLAUDIA CRISTINA. PRODUCCIÓN BIOCATALÍTICA DE ALCOHOL PERÍLICO UTILIZANDO CÉLULAS COMPLETAS DE Aspergillus niger DSM 821. Rev.Bio.Agro [online]. 2014, vol.12, n.1, pp.36-46. ISSN 1692-3561.

In this research, biotransformation of (R)-(+)-limonene for production of perillyl alcohol (POH) at lab scale was studied using whole cells of Aspergillus niger DSM 821. Effects of fungal growth phase, inductive effect of the substrate, pH, type of biotransformation medium and (R)-(+)-limonene concentration on both biotransformation selectivity and yield were evaluated. Using Malt Extract Agar (MEA) as culture medium at 28°C, it was possible to achieve higher specific growth rate of the fungi. Moreover, by using of Malt Yeast Extract Broth (MYB) and 12 mM of (R)-(+)-limonene as substrate in a liquid reaction medium at pH 5,0, it was obtained 246 mg/L of POH. This POH production was 1,9 and 3,1 times higher than yields obtained in liquid medium constituted by Yeast and Malt Extract, Peptone and Glucose (YMPG) and Broth Yeast Extract and Glucose (YG), respectively. Higher concentrations of POH (405 mg/L) were obtained by adding 50 mM of limonene in the exponential phase (at 72 h) of A. niger DSM 821 grown in MYB at pH 5,0, 28°C, 300 rpm and 6 days of biotransformation. Other by-products, such as: limonene-1,2-diol, linalool, carvone, phenyl ethanol and ethyl esters of palmitic, oleic and linoleic acids, were also obtained

Palabras clave : Biotransformation; Bioconversion; (R)-(+)-limonene; Aspergillus niger; Biotransformación; Bioconversión; (R)-(+)-limoneno; Aspergillus niger.

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