Abstract

DOTOR-ROBAYO, MÓNICA YADIRA et al. ANALY SIS OF GENETIC DIVERSITY OF BLACKBERRY (Rubus spp.) IN THE DEPARTMENT OF BOYACÁ. Rev.Bio.Agro [online]. 2016, vol.14, n.2, pp.10-17. ISSN 1692-3561.  https://doi.org/10.18684/BSAA(14)10-17.

A simple of 21 Rubus spp genetic ecotypes were characterized using markers Random Microsatellite RAMs. The seven primers produced a total of 160 polymorphic bands with molecular weights between 350 and 1500 Kb. RAMs analysis at a level of 55% similarity distinguished the population into five groups generally agree to the site where the ecotypes were collected. The number of polymorphic loci ranged from 16 (ACA) to 27 (CGA). For the total population the percentage of polymorphic loci and the expected average heterozygosity (He) were 88% and 0,29, respectively, much lower than values found in other genetic diversity studies in the genus Rubus. Therefore, strategies must be found to increase genetic variability. The coefficient of genetic differentiation (Fst) obtained in evaluating ecotypes Rubus, with seven RAMs microsatellite markers was 0,29 with a standard deviation of 0,03, values showing high genetic differentiation, which is associated with the level of population structure it tends to stabilize.

Keywords : Genetic markers; Microsatellites RAMs.

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