Abstract

JURADO GAMEZ, HENRY; ROMERO BENAVIDES, ALEJANDRO  and  MORILLO-GARCES, ALEXANDER. KINETIC, TEST OF GROWTH AND INHIBITION EFFECT Lactococcus lactis ON Yersinia pseudotuberculosis. Rev.Bio.Agro [online]. 2016, vol.14, n.2, pp.18-28. ISSN 1692-3561.  https://doi.org/10.18684/BSAA(14)18-28.

Lactic acid bacteria have demonstrated a high ability to inhibit pathogenic microorganisms, which improve knowledge of such microorganisms is important, for this, the kinetics was determined, growth and the inhibition effect of Lactococcus lactis on Yersinia pseudotuberculosis. The research was conducted at the University of Nariño, by susceptibility testing in all strains; in vitro inhibition of Lc. lactis and supernatant on bacterial pathogen; gastrointestinal lactic strain testing (gas production and catalase, bile, bile salts and 2 temperatures), growth kinetics and HPLC determination of peptides in the supernatant. dicloxacillin resistance was found in both strains. Lactic strain and the supernatant inhibited Y. pseudotuberculosis. growths and 3,9x10¹⁰ 3x10¹¹ CFU/150 uL to 3 to 5% bile salts, 3x10¹¹ 5x10¹² and CFU/150 µL 1 and 2% bovine 3x10¹³ and 3x10¹² bile and CFU/150 µL was found 38 and 45°C. The logarithmic phase of Lc. lactis was found at 3 hours with values 6,4x10¹² CFU/150 µL. The VAL-TIR-VAL peptide was found in the supernatant. It is concluded that Lc lactis shows probiotic characteristics in in vitro conditions.

Keywords : Probiotics; Antibiotics; Fermentation; Antagonism.

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