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Biotecnología en el Sector Agropecuario y Agroindustrial

versão impressa ISSN 1692-3561

Resumo

CASTILLO-SALDARRIAGA, CARLOS RAFAEL  e  VELASQUEZ-LOZANO, MARIO ENRIQUE. EFFECT OF DILUTE SULFURIC ACID PRETREATMENT ON THE ENZYMATIC HYDROLYSIS OF Panicum maximum. Rev.Bio.Agro [online]. 2018, vol.16, n.1, pp.68-79. ISSN 1692-3561.  http://dx.doi.org/10.18684/bsaa.v16n1.626.

Panicum maximum is a perennial grass with a high potential to be used in ethanol production due to drought and solar radiation resistant combined with a high biomass productivity. Within the unit operations used for the ethanol production, the pretreatment is the most energy demand operation, surpassed only by distillation. Therefore, operation parameters should be determined to achieve the intensification of it. In this study, the effects of two dilute sulfuric acid pretreatment parameters were evaluated using P. maximum: acid and solid concentration. Biomass was pretreated at 130oC and 60 min with different sulfuric acid concentrations (0,5; 1 and 1,5% w/v) and solid loading (5; 8,5 and 12% w/w). Later, pretreated biomass digestibility was evaluated through enzymatic hydrolysis. The selected pretreatment condition was 1,5% w/v acid concentration and 12% w/w solid concentration recovering 58,26 ± 0,62%. After 48-h enzymatic hydrolysis a reducing sugars concentration of 16,76 g/L was obtained.

Palavras-chave : High solids loading; Reducing sugars; Lignocellulosic biomass; Digestibility; Solids recovery.

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