Abstract

GALLO-GARCIA,, YULIANA; SIERRA-MEJIA,, ANDREA; GUTIERREZ,, PABLO-ANDRÉS  and  MARIN-MONTOYA,, MAURICIO. Prevalence of five RNA viruses in potato seed-tubers grown in Antioquia (Colombia). Rev.Bio.Agro [online]. 2021, vol.19, n.1, pp.66-78. ISSN 1692-3561.  https://doi.org/10.18684/bsaa(19)66-78.

Potato (Solanum tuberosum L.) is one of the agricultural products with highest internal demand in Colombia. However, this crop is affected by a virus complex that have a dramatic effect in yields and on the quality of tuber-seeds. In this work we tested the prevalence of five RNA viruses (PVY, PVS, PVX, PLRV and PYVV) in tuber-seed sprouts and their corresponding plantlets using real-time RT-PCR. Tuber-seeds were obtained from commercial (TC) and informal seed (TNC) distributors. Our results suggest high levels of infection with all five viruses in seed potatoes. Mixed infections, with up to four different viruses, were found with high frequency in certified or registered seed tubers. PVY (74%) and PVS (36%) were the most prevalent viruses using leaf tissues. The combination PVY+PLRV (24%) was the most common mixed infection in commercial tuber-seeds. In non-commercial seeds the most prevalent mixed infections were PVY+PYVV+PVS and PVY+PVS+PLRV at 10%. The phylogenetic affinity of all viruses under study was determined by partial sequencing of their CP genes, which confirmed their close relationship with previously characterized Colombian strains. These results underscore the urgent need to improve the health status in potato seed-tubers used by farmers in Colombia.

Keywords : RT-qPCR; Sequencing; Solanaceae; Plant virology; Potato viruses.

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