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Biotecnología en el Sector Agropecuario y Agroindustrial
Print version ISSN 1692-3561
Abstract
PAZ-DIAZ,, HÉCTOR-JULIO et al. Tannin extract from guanacaste tree fruit (Enterolobium cyclocarpum) as a tanning agent for European rabbit skin (Oryctolagus cuniculus). Rev.Bio.Agro [online]. 2021, vol.19, n.1, pp.180-190. ISSN 1692-3561. https://doi.org/10.18684/bsaa(19)180-190.
Tannins are secondary metabolites produced by guanacaste tree fruit that can form complexes with other macromolecules and increase the preservation of animal skins after the tanning process. This work focused on the extraction and quantification of vegetable tannins in guanacaste tree fruit (Enterolobium cyclocarpum) for its potential application as a tanning agent for European rabbit skin (Oryctolagus cuniculus). Extractions were performed using 70% aqueous ethanol, methanol and acetone solutions and then quantified by a modified Folin Ciocalteu method using tannic acid as the standard. Finally, the tanning capacity in rabbit skin was evaluated by the physical properties of the leather. It was found that the highest concentration of tannic acid equivalents (TAE) was obtained for extraction with 70% ethanol (1,42 g TAE / L) and 70% acetone (1,49 g TAE/L), respectively. These extracts were used to obtain the leathers according to standards for these products, passing only 3 out of 5 stability and resistance tests under official standards. These extracts are a potential substitute for chromium salts, allowing tanning processes with less environmental impact.
Keywords : Leather; Tanning; Antioxidants; Preservative; Enterolobium cyclocarpum; Oryctolagus cuniculus.