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Biotecnología en el Sector Agropecuario y Agroindustrial
Print version ISSN 1692-3561
Abstract
RAMIREZ-AYALA,, WILDER; GUEVARA-BRAVO,, CARLOS-ALBERTO and RODRIGUEZ-ESPINOSA,, JHON-ALEXANDER. Ganoderics acids from Ganoderma sp, Ganoderma lucidum and exhausted substrate. Rev.Bio.Agro [online]. 2021, vol.19, n.1, pp.206-216. ISSN 1692-3561. https://doi.org/10.18684/bsaa(19)206-216.
Ganoderma genus are recognized worldwide for their medicinal properties and their national and international market is continuously growing. In order to investigate the commercial potential of our fungal diversity, the contents of bioactive compounds, known as Ganodic Acids (A.G.), were determined from three sources of fungus. Of the fruiting bodies of two varieties of Ganoderma sp and Ganoderma lucidum (wild and commercial). The wild variety was collected in the municipality of Quimbaya Quindío, the commercial, certified, was developed on agro-industrial plantain waste and the spent substrate were obtained after the harvesting of the commercial variety. The three samples obtained in triplicate, were dried and subjected to extraction with supercritical fluids and ultrasound. The concentration of A.G. was determined by Liquid Chromatography (HPLC), by the method of normalization of areas, using as standard Ganoderic Acid A. The method of extraction by ultrasound was the most efficient in all cases. The highest content of A.G., was found in the wild strain (266 μg/g), approximately 30% more than in the commercial variety, although considerably less than international studies. It is possible to conclude that the wild variety would have the potential to become a commercial source of A.G.
Keywords : Culture; Basidiomycetes; Wild strain; Extraction; Secondary metabolites; Chromatography; Ganoderma lucidum..