Abstract

SALAMANCA-GROSSO, GUILLERMO,; OSORIO-TANGARIFE, MÓNICA-PATRICIA,  and  CABRERA-MONCAYO, JESUS ANTONIO,. Propolis from Nariño: Physicochemical properties and biological activity of Propolis. Rev.Bio.Agro [online]. 2022, vol.20, n.1, pp.152-164.  Epub Dec 30, 2021. ISSN 1692-3561.  https://doi.org/10.18684/bsaa.v20.n1.2022.1863.

Propolis is natural product processed by honey bees from plant exudations with a resinous and rubber-like consistency. This work examines the physicochemical properties, sensory attributes, and biological activity of propolis ethanol extracts from three different biogeographic zones in department of Nariño, Colombia. Samples of raw propolis obtained from each area were compared, and their alcoholic extracts studied through separation methods and spectroscopic techniques. Biological activity was evaluated using Staphylococcus aureus and Escherichia coli strains. Chromatographic analysis revealed the presence of secondary metabolites with potential biological activity. Infrared spectrum and chromatograms displayed differences between group samples, identified as type -O and -B. Phenolic fraction exhibited significant variations on flavones and flavanols. Oxidation rates were lower than 5 seconds. Evaluated ethanolic extracts exposed greater sensitivity to S. aureus under the Kirby-Bauer technique. Present research contributes towards expanding knowledge about natural-origin products like propolis on such Colombian regions.

Keywords : Biological activity; Phenols and flavonoids; Colombian propolis; Physicochemical properties; HPTLC; LC-ESI(−)-MS/MS.

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