Revista Ciencias de la Salud
versão impressa ISSN 1692-7273
FONSECA, Dora et al. Analysis of Urine Samples for the Molecular Detection of Infectious Diseases. Application to the Identification of Human Cytomegalovirus. Rev. Cienc. Salud [online]. 2005, vol.3, n.2, pp. 136-147. ISSN 1692-7273.
Molecular biology methods like Polimerase Chain Reaction (PCR) has been used for diagnosis of infectious diseases. Until today, the identification methods are based mainly on cultures and serology due to their sensibility and specificity, but they are expensive and time consuming. Urine samples constitute an alternative, noninvasive, method of obtaining DNA for the accomplishment of molecular Biology analysis. Methodology: implementation of a strategy to obtain DNA from urine samples. Samples were taken from children in daycare centers, to document the presence of inhibitors, PCR amplification of genes of human Cytomegalovirus (HCMV) was done. Results: In 27.1% of the analyzed samples, specific amplification for HCMV was demonstrated. No viral significant differences were found in the three layers, although it was present in the bands. Conclusion: The inhibitor absence was verified using PCR by amplificating the gene of the B-globine. A molecular methodology for the HCMV identification was standardized, which can be applied in prenatal diagnosis of congenital infection.
Palavras-chave : Cytomegalovirus infection; diagnosis; urine; inhibitors; extraction; DNA; Ethylene glycol.