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Revista EIA
versão impressa ISSN 1794-1237versão On-line ISSN 2463-0950
Resumo
RODRIGUEZ, Olga Viviana e HANSSEN, Henry. OBTENCIÓN DE DEXTRANO Y FRUCTOSA, UTILIZANDO RESIDUOS AGROINDUSTRIALES CON LA CEPA Leuconostoc mesenteroides NRRL B512-F. Rev.EIA.Esc.Ing.Antioq [online]. 2007, n.7, pp.159-172. ISSN 1794-1237.
Due to biotechnological potential that presents the agro-industrial remainders, the present work was developed about obtaining oligosaccharides using like raw material remainders of orange peels, pineapple peels, and panelera cane slowness, at level laboratory. An experimental design was used to evaluate type and concentration of substrate and process temperature at three levels, accordingly the greatest amount of dextran and fructose produced. All volumes worked were 100 mL and 250 mL. In the final phase, the result was a production of dextran 3.4 g/L and fructose 5.04 g/L, using orange peels as substrate, temperature at 30 °C and concentration of substrate of 20 g/L, determining for each sample: consumption of substrate, biomass, and amount of products. The growth of the microorganism was observed; correspondingly to a substrate without addition of nutrients with a favorable adaptation at medium supplied. Finally, an experimental characterization was developed, and it was concluded that technical grade dextran can be obtained used in food like thicker and waste water treatment.
Palavras-chave : agro-industrial remainders; biopolymers; dextran; fructose; Leuconostoc mesenteroides.