Abstract

JUTINICO SHUBACH, AP; MALAGON GARZON, Esp  and  SANCHEZ MORA, RM. Cultivation of the cell line hep-2: dubbing of population and coloring with Giemsa. Nova [online]. 2013, vol.11, n.20, pp.23-33. ISSN 1794-2470.

Cell cultures have become essential tools for basic research. They are applied in immunology, virology, molecular biology, genetic engineering and pharmacology, among other areas. They are also used in pharmaceutical industrial processes, in techniques of clinical diagnostic, and to study tissue transplantation. In bacteriology, these crops allow us to confirm an infection, assess the efficiency of antimicrobials, carry out studies of infectivity, investigate on new species, obtaining a large number of microorganisms non-arable to optimize techniques, and to examine the relationship between the host cell and intracellular microorganisms (bacteria, viruses, and parasites). The HEp-2 cell line (Human epidermoid cancer cells) is used in studies of infection with different bacteria, including Chlamydia trachomatis (CT), in order to determine the mechanisms by which the pathogen survives in the host cell. It is also used to observe the action of antimicrobial peptides and extracts to combat the infection caused by the bacterium. For this study, growth curves of the HEp-2 cell line were carried out with DMEM-F12 and MEM media. In addition, the staining with Giemsa was standardized, and the population dubbing was calculated with different inocula for assessing the development of the cultured cell line and select the optimal conditions for future tests of infection with intracellular parasites, in particular with CT serovar L2.

Keywords : cell culture; cell line HEp-2; chlamydia trachomatis; dubbing population; infection.

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