Abstract

CORTES CORTES, Liliana Jazmín et al. Optimization of Polymerase Chain Reaction for the detection of the B1 gene of T. gondii. Nova [online]. 2014, vol.12, n.22, pp.137-142. ISSN 1794-2470.

Objective: This study aimed at optimizing the amplification conditions of the B1 gene (35 copies in the ge ­nome) of T. gondii in cases of possible brain toxoplasmosis. Materials and methods: DNA was extracted from the peritoneal exudate of mice inoculated with the RH strain of T. gondii. Total exudate volume was 17 mL with a concentration of 1x10⁷ parasites/mL. PCR conditions for the B1 gene were further optimized.Results: Serial dilutions from 1x10⁶ to 1x10^-1 parasites/mL were needed for amplifying a fragment of 132 bp . This set the detection limit for 1 T. gondii tachyzoite.

Keywords : toxoplasmosis; optimization; polymerase chain reaction; B1 gene detection.

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