Abstract

PINILLA B, Gladys et al. Determination of genes, 16S ADNr, polA, and TpN47, in the detection of Treponema pallidum subsp. pallidum in the diagnosis of congenital syphilis. Nova [online]. 2015, vol.13, n.24, pp.17-25. ISSN 1794-2470.

Objective. To compare the behavior of the three target genes (16S rDNA, polA, and TpN47) for the detection of T. pallidum subsp. Pallidum. Methods. Molecular techniques such as polymerase chain reaction were used on samples of an umbilical cord. Molecular targets of the microorganism were amplified by means of conventional PCR, nested PCR and real-time PCR. Results. Similar results for the three genes were obtained by conventional PCR; but in the case of nested PCR and real-time PCR, the gen TpN47 exhibited higher sensitivity in comparison to the genes polA and 16S rDNA. In conclusion, the gen TpN47 can be used as a molecular target for the prompt diagnosis of congenital syphilis through nested PCR and real-time PCR due to its high sensitivity and specificity shown in this study.

Keywords : Congenital syphilis; nested PCR; real-time PCR; Treponema pallidum subsp. pallidum.

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