SciELO - Scientific Electronic Library Online

 
vol.14 número25Blattella germánica (Blattodea: Blattellidae) as potential mechanical vector of health-care associated infections in a hospital in Villavicencio (Meta-Colombia) índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

  • Em processo de indexaçãoCitado por Google
  • Não possue artigos similaresSimilares em SciELO
  • Em processo de indexaçãoSimilares em Google

Compartilhar


Nova

versão impressa ISSN 1794-2470

Resumo

ORDONEZ VASQUEZ, Adriana; JARAMILLO GOMEZ, Lorenza; IBATA, Margie  e  SUAREZ-OBANDO, Fernando. China ink in adherents ceil in culture. Nova [online]. 2016, vol.14, n.25, pp.9-17. ISSN 1794-2470.

Objective. To implement the use of China ink, as an alternative technique, to display morphological changes in cellular wall and cellular matrix in adherent living cells in culture flask, before and after exposure to a toxic substance. Methods. China Ink was implemented as a contrast technique in optical microscopy, by comparing observed sharpness (proper appreciation of edge structures) from cultures of bone marrow stem cells (CTMO) exposed to the toxic glycoalkaloid α-solanine. The sharpness differences were compared among the diverse treatments with Fisher's exact test. Results. China Ink staining clearly helps to identify abnormal phenotypic changes, secondary to cytotoxic exposure in adherent cells in culture (p <0.0001). Conclusions. China Ink is useful in clearly displaying the CTMO cells and the effects of the α-solanine glycoalkaloid in adherent cells in culture. It is a simple method that contributes to the understanding of the effect of various substances on CTMO in culture.

Palavras-chave : China Ink; Solanine; Stem Cells; In Vitro; Optical Imaging.

        · resumo em Espanhol     · texto em Espanhol     · Espanhol ( pdf )

 

Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons