Abstract

PACHON MEZA, Laura Cristina  and  BARRERA AYALA, Sandra Milena. Persistent non-specificity in loop-mediated insothermal amplication for malaria diagnosis. Nova [online]. 2016, vol.14, n.26, pp.29-33. ISSN 1794-2470.

Objetive. The present study describes the assay performed to standardize a malaria diagnostic DNA test, called loop-mediated isothermal amplification (LAMP). Methods. Extractions from 48 Plasmodium-infected blood samples (22 P.falciparum-positive samples, 20 P. vivax-positive samples and 6 negative control samples). Results. The data show false positives, unspecific amplifications that demonstrate the low specificity of the test. We propose to study the amplicons obtained by restriction fragment and sequencing to investigate the underlying cause of the problem.

Keywords : malaria; doop-mediated; isothermal; amplification (LAMP); LAMP specifity; P. falciparum; P. Vivax.

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