Resumo

PINILLA B, Gladys; CRUZ B, Claudia Andrea  e  NAVARRETE O, Jeannette. Molecular diagnosis of SARS-CoV-2. Nova [online]. 2020, vol.18, n.spe35, pp.35-41.  Epub 15-Jan-2021. ISSN 1794-2470.  https://doi.org/10.22490/24629448.4184.

COVID-19 diagnosis is based on both clinical aspects and screening tests. However, clinical symptoms and signs in infected patients are highly atypical; hence, molecular tests are essential for diagnosis. RT-qPCR is carried out at BSL II level laboratories; the main molecular targets for viral detection are E gene (envelope), and RdRP gene (RNA-dependent RNA polymerase). False negatives in this diagnosis are due to sample quality and quantity, transport conditions, storage and handling before and after extraction (RNA is heat-labile and RNases are abundant); infection phase; virus mutations and presence of CRP inhibitors. Taking into account analytical sensitivity of RT-qPCR (5.2 copies of RNA / reaction) and the fact that once RNA it is extracted, it progressively degrades and affects test diagnostic sensitivity, a new sample -specifically taken from the lower respiratory tract in order to increase viral load- is recommended in the abovementioned cases. Timely diagnosis allows optimizing management (isolation and treatment), patient monitoring, implementing prevention and control measures as well as epidemiological surveillance of the disease.

Palavras-chave : diagnosis; RT qPCR; SARS virus; coronavirus.

        · resumo em Espanhol     · texto em Espanhol     · Espanhol ( pdf )