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Nova
Print version ISSN 1794-2470
Abstract
BOLIVAR TORRES, Hermes Hernán et al. Culture xerophile microorganisms from the Tatacoa semiarid zone (Colombia). Nova [online]. 2021, vol.19, n.36, pp.19-30. Epub Feb 12, 2022. ISSN 1794-2470. https://doi.org/10.22490/24629448.5282.
Introduction.
Xerophilic microorganisms have become more relevant for conducting research related to their adaptive mechanisms against water stress, as well as the characterization and identification of their habitats. In Colombia, semi-arid and desert areas such as the Tatacoa desert have been little studied at the microbial level.
Objective.
Isolation and characterization of cultivable xerophilic microorganisms from the soil of the semi-arid zone of Tatacoa, in the department of Huila (Colombia).
Material and methods.
For this, samples were collected in the sectors locally called Cuzco and La Victoria, which were processed for the isolation of xerophilic microorganisms in selective M40Y medium for subsequent macro and microscopic characterization, as well as evaluation by biochemical tests for the use of substrates.
Results.
29 morphotypes were isolated among which it was possible to differentiate: Gram-positive bacilli and cocci present exclusively in the Cuzco sector and branched filamentous bacteria (Actinobacteria) only in the La Victoria sector. The presence of the genera Streptomyces, Micrococcus and Corynebacterium was established.
Conclusions.
The presence of microorganisms related to the mentioned genera will allow us to understand the possible interactions that occur in this ecosystem, which will contribute to the development of this place as a possible analogue of studies for the search for life on other planets such as Mars. In addition, promote more detailed studies where microorganisms that are useful for different biotechnological processes can be recovered.
Keywords : bacteria; desiccation; arid zone; exobiology; Colombia; Mars.