Abstract

GUTIERREZ, Luz Adriana  and  AGUDELO, Divier Antonio. Growth control In Vitro on Gram - negative and Gram - positive Strains that produce mastitis. Rev. Lasallista Investig. [online]. 2009, vol.6, n.1, pp.67-74. ISSN 1794-4449.

Introduction. Mastitis is a disease that causes loses worldwide, especially in regions with an extensive milk production. It is caused by microorganisms that damage the udders, producing a bacterial resistance to antibiotics with the pass of time and, in consequence, re-emerging infections. A solution to this problem is the use of different monosaccharide and disaccharide loads on the microorganisms that produce mastitis. Objective. To evaluate the effect of monosaccharide and disaccharide different concentrations on microorganisms that cause mastitis. Methodology. Escherichia coli and Staphylococcus aureus samples were taken from milks coming from cows in Santa Rosa de Osos. The growth curves of the microorganisms were measured to identify the moment of their stationary phase. The concentration of microorganisms for the evaluation with the corresponding sugars was 10⁶/ml inoculated in 9ml of those sugars at the different concentrations. Their inhibition was determined by the use of spectrophotometry, approximately 20 hours after their incubation. Results. Staphylococcus aureus is susceptible to the action of sugars like sucrose and glucose, while Escherichia Coli has a higher tolerance. The variance analysis of two factors showed that there are significant differences in each treatment for both Escherichia Coli and Staphylococcus aureus. Conclusion. A bacteriostatic effect of sucrose and glucose on the growth In vitro of Staphylococcus aureus and Escherichia Coli in concentrations above 30% and 35 %, respectively, was demonstrated.

Keywords : Bovine mastitis; Gram positive strains; Gram negative strains.

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