Abstract

MANJARRES, Katerine; CASTRO, Adriana  and  RODRIGUEZ SANDOVAL, Eduardo. Production of locase using Pleurotus ostreatus on plantain peel and sugar cane bagasse. Rev. Lasallista Investig. [online]. 2010, vol.7, n.2, pp.9-15. ISSN 1794-4449.

Introduction. Plantain peel for industrial purposes represents from 35% to 40% of the total fruit weight, becoming a considerable quantity of waste which could be used to produce enzymatic complexes. Objective. To investigate the potential green and mature plantain peel of "Dominico-Hartón" as a supportsubstrate to produce laccase extracellular enzyme by the use of Pleurotus ostreatus fungus. Materials and Methods. Pleurotus ostreatus fungus was cultivated in different mixtures, as follows: Green plantain peel (GPP), mature plantain peel (MPP) green plantain peel-cane bagasse (50/50) (GPPCB), mature plantain peel-cane bagasse (50/50) MPPCB). Fermentation units were kept at pH 4.8, 28°C and a 63% humidity. The determination of the laccase enzyme was carried out by ABTS oxidation. Results. In the substrates used, a maximum laccase activity was achieved in the 24^th day, except in the mature plantain treatment, which achieved it in the 34^th day. MPPCB substrate had the highest production of laccase with 19.4 U/mL concentration. Conclusion. Plantain peel can be a good substrate when is supplemented with other lignocellulosic wastes, such as sugar cane bagasse. Laccase can be used as a morphogenetic indicator because it shows the maximum growth of the mycelium.

Keywords : plantain cane; cane bagasse; Pleurotus ostreatus; laccase.

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