Abstract

ZAMBRANO ARTEAGA, Juan Carlos; ECHEVERRI ZULUAGA, Julián  and  LOPEZ HERRERA, Albeiro. Characterization of the genetic polymorphism of the BoLA DRB3.2 gene in Holstein and Bonholstein cows from a dairy herd in Antioquia. Rev. Lasallista Investig. [online]. 2016, vol.13, n.1, pp.76-84. ISSN 1794-4449.

Introduction. DRB3 gene of the bovine leukocyte antigen (BoLA) has been widely studied, given the fact that its allelic variations have been associated to infectious diseases and production characteristics. Objective. To determine the frequencies of the alleles of the exon 2of the BoLA DRB3.2 gene obtained by means of the PCR-RFLP technique in Holstein y BONxHolstein (BxH) cows from a dairy herd in Antioquia, Colombia. Materials and methods. 101 cows were genotyped, 76 were Holstein and 25 of the BON X Holstein genetic cross (BxH) for the exon 2 of the DRB3 gene of the bovine leukocyte antigen (BoLA). The BoLA DRB3.2 alleles were identified by means of the PCR-RFLP technique using the Rsal, Haelll and BstYI restriction enzymes and were confirmed with a sequencing process. Results. 27 BoLA DRB3.2 alleles were identified with a frequency ratio from 0.007 to 0.151 in the Holstein cows and from 0.02 to 0.20 in BxH. The four most frequent alleles for Hostein cows were: BoLA DRB3.2 ^* 23, 22, 24 and 16, with an accumulated frequency of 0.506, and the four most frequent alleles in BxH were: BoLA DRB3.2^*23, 24, 20 and 37, with an accumulated frequency of 0.54. Conclusions. The BoLA DRB3.2 gene was highly polymorphic in both genetic groups (Holstein and BxH) and also in both groups there was a similar allelic profile.

Keywords : PCR-RFLP; Bovine; polymorphism; Major Histocompatibility Complex.

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