Abstract

SIMANCAS-ESCORCIA, Victor  and  DIAZ-CABALLERO, Antonio. Impact of bovine fetal serum concentration on dental epithelial cells. Entramado [online]. 2019, vol.15, n.1, pp.276-284. ISSN 1900-3803.  https://doi.org/10.18041/1900-3803/entramado.1.5420.

Dental enamel, the hardest tissue in the human body is formed by the differentiation of epithelial cells known as ameloblasts. Recently rat incisor dental epithelial cells have been used as a cellular model of pathophysiological events during tooth enamel formation. However depending on the events studied, it is necessary to understand the behavior of these cells, particularly when the concentration of Bovine Fetal Serum (FBS) varies. The purpose of this work is to evaluate the impact of FBS concentration on the growth, proliferation, and survival of dental epithelial cells. Dental epithelial cells were cultured in DMEM/F12 culture medium in the absence and presence of 10% FBS. Morphological and immunohistochemical observations of actin, vimentin, and fibronectin were performed. The results confirm that the absence of FBS negatively affected the proliferation of dental epithelial cells but maintained an optimal expression of actin and vimentin. An alteration in the expression of fibronectin in the cells treated in the absence of FBS was identified. In conclusion, the lack of FBS dramatically decreased the survival, proliferation, and expression of fibronectin in rat dental epithelial cells.

Keywords : Ameloblasts; dental enamel; culture media; culture media serum-free; cell proliferation (Source: Decs).

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