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Entramado
versión impresa ISSN 1900-3803versión On-line ISSN 2539-0279
Resumen
PAYAN GONZALEZ, Andrey; JURADO OREJUELA, Diana Maritza y GARZON LANCHEROS, Luz Myriam. Are modified manual methods valid to establish Erythrocyte Sedimentation Rate (ESR) in clinical laboratories?. Entramado [online]. 2020, vol.16, n.1, pp.230-238. ISSN 1900-3803. https://doi.org/10.18041/1900-3803/entramado.1.6088.
Four methods were statistically evaluated for their validity to determine the alternative Erythrocyte sedimentation rate to that of Westergren, which was taken as the “gold standard”. The methods evaluated were Wintrobe (WB), Wintrobe inclined (WI) at 45° and two capillary micromethods, one vertical (MM) and one inclined at 45° (MMI). A total of 419 samples were processed by the five methods. Concordance (C), sensitivity (S), specificity (E), positive predictive value (PPV) and negative predictive value (NPV) were evaluated. The results for S, E, PPV, NPV and C were: 93.8%, 93.6, 98.8%, 72.8% and 71% for WB; 86.3%, 85.7%, 97.2%, 52.4% and 54% for WI; 94.6%, 66.6%, 94.1%, 71.4% and 54% for MM and 91.9%, 72.4%, 94.8%, 60.8% and 55% for MMI. The kappa index showed “good” agreement between the Westergren method and the Wintrobe method and “moderate” agreement with the WBI, MM and MMI methods. The results of the present study show that the Wintrobe method is reliable for use in the clinical laboratory compared to the Westergren method.
Palabras clave : Erythrocyte Sedimentation Rate; Westergren; gold standard; concordance; validity.