Resumo

URIBE VALDERRAMA, Rubén; ARANGO RODRIGUEZ, María Elena; RENDON ALVAREZ, Laura  e  ACEVEDO NARANJO, Carlos Mauricio. Evaluaton of canine semen that has gone through a freezing and thawing process with different glycerol concentrations as a crioprotector. Ces. Med. Vet. Zootec. [online]. 2011, vol.6, n.1, pp.21-30. ISSN 1900-9607.

The study of biotechnology used in canines for reproductive purposes, is a of the principal research study points to the area of Veterinary Medicine in last years²⁸. In the objective to evaluate the effect of three different concentrations of glycerol on canine semen, to subjected cryo-preservation process, were obtained using manual manipulation, 12 semen samples of male Labrador clinically healthy and subject to the same driving conditions. Each sample was divided in four treatments (TI: control, T2: 4%, T3: T4 6% and 8% glycerol), to which they evaluated the volume, pH, motility, concentration, morphology and vitality, respectively. Then in the analysis were determined after thawing seminal differences and its relationship to the concentration of cryoprotectant. From the results obtained in this project are significant differences between the variables morphology, mortality and concentration, also established that T2 (4% glycerol) and T3 (6% glycerol) provide better results for freezing semen canines.

Palavras-chave : Canine semen; cryopreservation; glycerol; seminal viability.

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