Abstract

URUBURU GOMEZ, Mónica; BEDOYA BLANDON, Juan Carlos  and  VELASQUEZ TRUJILLO, Luz Elena. Indirect ELISA for diagnosing bovine fasciolosis from milk samples. Ces. Med. Vet. Zootec. [online]. 2013, vol.8, n.2, pp.93-100. ISSN 1900-9607.

Abstract We standardized an ELISA test for the detection of anti-F. hepatica antibodies in bovine milk. A total of 80 samples were used, of which 44 were used as positive controls and 36 as negative controls. Milk samples from bovines presenting paramphistomidae eggs in feces were used as cross-reactivity controls. Sensitivity and specificity of the diagnostic test were 80 and 72,2%, respectively, with 95% confidence interval. These results are comparable with those reported in studies conducted in other countries. We propose this standardized test as an alternative diagnostic and screening tool in epidemiological studies of fasciolosis.

Keywords : dairy herds; epidemiology; Fasciola hepatica; inmunodiagnosis.

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