Abstract

MUNOZ GARCIA, Fabián Gerardo; VILLAMARIN, Julián Antonio  and  LONDONO FRANCO, Luis Fernando. Chromosomal differences in Sabaletas (Brycon henni) from the upper basins of the Cauca and Patia rivers. Ces. Med. Vet. Zootec. [online]. 2016, vol.11, n.1, pp.62-71. ISSN 1900-9607.

This research chromosomically compared Sabaletas (Brycon henni) from the upper basins of the Cauca and Patia rivers in the Cauca department. For the study 6 specimens were captured in each site and transported to the Genetic toxicology laboratory of the Universidad del Cauca. Once there, kidney cells were extracted and cultivated, spreads of cells in metaphase were done, pictures were taken with an optical microscope, and the modal chromosome number and types of chromosomes were determined according to the position of the centromere, manually and using an assisted segmentation system for image processing. The results showed that Brycon henni has a chromosomal number of 2n=52, and two karyological formulas specific to each of the basins studied: Brycon henni from the Cauca river had the kariotype formula 22m+16sm+14st, while from the Patia river basin the formula was 8m+10sm+26st+8t. These differences suggest that the specimens studied from both basins are probably in a speciation process due to geographical isolation. Moreover, no differences in the chromosome number were found between males and females on both basins. Additionally, the physico-chemical parameters of the water from the upper basins of the Cauca and Patia rivers have not shown significant changes that could affect the physiological conditions of these fishes.

Keywords : Chromosome set; fishes; karyological formula; modal number; metaphase.

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