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CES Medicina Veterinaria y Zootecnia
On-line version ISSN 1900-9607
Abstract
LOPEZ ROJAS, Luis Ernesto; PATINO-CADAVID, Laura; LOPEZ HERRERA, Albeiro and ECHEVERRI ZULUAGA, José Julián. Genetic variability in six SNPs of CAPN1, CAST and LEP genes of brahman bulls in cattle farms on colombian tropical lowlands. Ces. Med. Vet. Zootec. [online]. 2017, vol.12, n.2, pp.88-102. ISSN 1900-9607. https://doi.org/10.21615/cesmvz.12.2.2.
The CAPN1 and CAST genes encodes μ-calpain and calpastatin, both genes participate on degradation of muscle fibers after rigor mortis; in addition the LEP gene codes for leptin, which regulates the fat deposition in muscle. These genes shows SNPs related to tenderness and juiciness of meat and productive characteristics of the carcass. In order to know the genetic potential of Brahman cattle the CAPN316, CAPN4751, CAST282, CAST2959, E2FB and E2JW SNPs were genotyped by PCR-HRM in 270 Brahman males from four different geographical areas, also the study of differentiation of population structure was made.
There was no difference (p> 0.05) in genotype and allele frequencies of these markers between subpopulations, the genotypic frequencies in the general population were: CAPN316 (CG = 0.078, GG = 0.922), CAPN4751 (CC = 0.289, CT = 0.104, TT = 0.606), CAST282 (CC = 0.285, CG = 0.448, GG = 0.267), CAST2959 (AA = 0.400, AG = 0.370, GG = 0.230), E2FB (CC = 0.633, CT = 0.356, TT = 0.011) and E2JW (AA = 0.985, AT = 0.010, TT = 0.004). The CAPN316, CAST282 and E2JW markers were found in HWE (p> 0.05), while CAPN4751 and CAST2959 showed Ho < He and in E2FB Ho > He. The F statistics values were: FIS = 0.153, FIT = 0.157 and FST = 0.005.
Was conclude that the Brahman herds studied present a slight degree of inbreeding and constitute a single population; all markers evaluated are polymorphic, except for E2JW, however the production of more tender and juicy meats could be possible, because the associated alleles with this characteristics were founds although in low frequencies.
Keywords : CAPN316; CAPN4751; CAST282; CAST2959; juicy; LEPE2FB; LEPE2JW; tenderness.
