Resumo

VELILLA MSC, Claudia; MARTINEZ MS, Juliana  e  GONZALEZ MSC, María Soledad. Standardization of Multiplex Real-Time PCR for the diagnosis of Feline Immunodeficiency Virus (FIV) and Feline Leukemia Virus (FeLV) in Felis silvestris catus. Ces. Med. Vet. Zootec. [online]. 2020, vol.15, n.1, pp.31-43. ISSN 1900-9607.  https://doi.org/10.21615/cesmvz.15.1.3.

Diseases such as Feline Immunodeficiency Virus (VIF) and Feline Leukemia Virus (VLFe) are highly prevalent in domestic cats, due to their ease of transmission. Clinical signs are similar to other infections causing difficulties in diagnosis, therefore, they should be analyzed by laboratory tests; Currently available tests have some disadvantages, a multiplex real-time PCR was standardized with Taqman probes, designed for detection of infection with VIF, VLFe or both. Sensitivity (0.53/0.26) and specificity (0.46/0.74) for VLFe and VIF respectively, were calculated with Broemeling's methodology, which considers that the reference test against which it is compared is not a Gold standard, according to these results it is necessary to increase the sample size; However, multiplex PCR is a very sensitive methodology, it was validated In silico form and presented congruent results when performed in vivo.

Palavras-chave : Molecular diagnosis; Feline Leukemia virus; Multiplex PCR; Feline Immunodeficiency virus..

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