Abstract

OSORIO, José Henry  and  FLOREZ, Jancy Darly. COMPARISON OF DIRECT AND PRECIPITATION METHODS FOR DETERMINING HDL CHOLESTEROL LEVELS IN LAYING HENS. Luna Azul [online]. 2014, n.38, pp.122-131. ISSN 1909-2474.

Objective: To compare a direct method vs a precipitation method for determining HDL cholesterol in laying hens. Methodology: Serum of forty 26 weeks old fasting laying hens was obtained from the Hy-Line W-36. Cholesterol in high density lipoproteins was measured by direct method based on a specific detergent along with N,Nbis (4-sulfobutyl)-m-toluidine, and through the precipitation method based on phosphotungstate + Mg²⁺. Results: The standard deviation mean ± for high density lipoproteins though direct and precipitation methods in mg/dl were: 63.1±14.3; 13.3±3.5 respectively. The P value for the F test is < 0.05 showing significant statistical difference between the two analyzed methods with a confidence level of 95%. Conclusions: It is feasible to use the direct method using a specific detergent and N,Nbis(4-sulfobutyl)-m-toluidine to determine high density lipoprotein cholesterol in laying hens, because this method is not altered by high levels of triglyceride.

Keywords : Detergente + DSBmT; lipids; lipoproteins; phosphotungstate.

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