Servicios Personalizados
Revista
Articulo
Indicadores
- Citado por SciELO
- Accesos
Links relacionados
- Citado por Google
- Similares en SciELO
- Similares en Google
Compartir
Revista Ingeniería Biomédica
versión impresa ISSN 1909-9762
Resumen
PINEDA MOLINA, Catalina y LONDONO PELAEZ, Carolina. OBTENCIÓN DE CÉLULAS MADRE DEL TEJIDO ADIPOSO Y SU POTENCIAL DE DIFERENCIACIÓN OSTEOGÉNICOADIPOSE TISSUE DERIVED MESENCHYMAL STEM CELLS, ISOLATION AND DIFFERENTIATION INTO THE OSTEOGENIC LINEAGE. Rev. ing. biomed. [online]. 2009, vol.3, n.5, pp.58-65. ISSN 1909-9762.
Adipose tissue is one of the main sources of stem cells readily available and with a high potential to differentiate into specific mesenchymal and non-mesenchymal lineages. Therefore, to standardize the collection of these cells, adipose tissue from surgical liposuctions and abdominoplasty was used. Methods of enzymatic and mechanical digestion were used to release the cells. With regard to cell morphology, the cells displayed a fibroblast-like spindleshaped morphology as cited by other authors. There was a higher efficiency in processing tissue from liposuctions compared with tissue from abdominoplasty. Likewise, the enzymatic digestion allowed faster initial cell adhesion than the mechanical digestion. Finally, calcium deposits were observed by alizarin red staining in cultures under osteogenic conditions, but were absent in cultures lacking osteogenic supplements. In conclusion, isolated adiposedderived stem cells were capable of osteogenic differentiation and therefore with potential applications in bone tissue engineering.
Palabras clave : Adipose tissue; Liposuction; Mesenchymal stem cells; Osteogenic differentiation.