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Revista Colombiana de Ciencias Hortícolas

Print version ISSN 2011-2173

Abstract

CRIOLLO E., HERNANDO; INSUASTI, KATHERINE  and  DELGADO, WILMER. In vitro regeneration of tamarillo (Solanum betaceum (Cav.) Sendt.). rev.colomb.cienc.hortic. [online]. 2016, vol.10, n.2, pp.252-261. ISSN 2011-2173.  http://dx.doi.org/10.17584/rcch.2016v10i2.5750.

ABSTRACT The tree tomato is a fruit of importance in Colombia, with a productivity limited by the presence of sanitary problems that are transmitted through the traditional methods of propagation, that force to look for more efficient methods for the obtaining of healthy plants. This study was carried out to evaluate different alternatives of micropropagation that would allow us to determine the most efficient option for the mass production of tamarillo plants (Solanum betaceum). Hypocotyls explants were evaluated under the effect of three auxins: AIA, ANA and 2,4-D in different doses, combined with BAP. The basic medium used was Murashige Skoog (1962). The largest average of number of shoots was obtained when using AIA 0.5 mg L-1 + BAP 3.0 mg L-1 with a value of 11.6 shoots. The best treatment for rooting of hypocotyls was AIA and the control without phytoregulators. Regeneration of complete plants was better to AIA 0.5 mg L-1, with an average of 36.9%. The best average for rooting of shoots obtained was obtained with AIA 1.0 mg L-1 + BAP 3.0 mg L-1 with 96.23% and with AIA 1.0 mg L-1 + BAP 1.5 mg L-1 (88.83%). The rooted plant were successfully transplanted into the soil with a 96.6% survival rate.

Keywords : benzylaminopurine; indoleacetic acid; naphthaleneacetic acid; 2.4-D; micropropagation; hypocotyls.

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