Abstract

DUQUE-ZAPATA, Juan Diego; MUNOZ, Jaime Eduardo  and  CHECA-CORAL, Oscar. Molecular characterization using SSR markers in 50 shrub pea genotypes (Pisum sativum L.) from the GRICAND Collection, Colombia. rev.colomb.cienc.hortic. [online]. 2019, vol.13, n.2, pp.208-218. ISSN 2011-2173.  https://doi.org/10.17584/rcch.2019v13i2.10177.

The pea (Pisum sativum L.) is one of the more important legume crops produced globally. We studied the structure and genetic diversity in a collection of 50 pea accessions with 16 simple sequence repeat (SSR) mar kers, whose average polymorphic information content (PIC) was 0.62. The SSR markers amplified a total of 28 alleles with an average of 4 alleles per locus, with locus AB71 and D21 amplifying the largest number of alleles (6). The observed heterozygosity (Ho) was 0.09±0.08 and the expected heterozygosity (He) was 0.42, indicating an elevated level of inbreeding (Fis = 0.60). The genetic relationships were inferred with a similari ty index (DICE) and a bayesian analysis (STRUCTURE), detecting 2 clusters for the genotypes, with a high similarity of the morphological characteristics of each genotype. The results of this study will be useful for the creation of future breeding programs.

Keywords : genetic diversity; genetic structure; pre-breeding; SSR markers; grain legumes; plant habit.

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