Abstract

RODRIGUEZ-MORA, DIANA MILENA; MARTINEZ-CABALLERO, LUZ NATALIA; BETANCOURT, MÓNICA  and  MORENO, ISABEL. Occurrence and molecular characterization of cucumber mosaic virus (CMV) in plantain orchards in Caldas and Risaralda departments of Colombia. rev.colomb.cienc.hortic. [online]. 2024, vol.18, n.3, e17841.  Epub Apr 05, 2025. ISSN 2011-2173.  https://doi.org/10.17584/rcch.2024v18i3.17841.

Cucumber mosaic virus (CMV) affects plantain (Musa AAB) orchards in most producing regions across the globe. This study aimed to determine the occurrence and characterize the CMV affecting plantain orchards in the departments of Caldas and Risaralda, Colombia. Leaf tissue samples were collected from six municipalities, then screened for CMV diagnosis using the enzyme-linked immunosorbent assay (ELISA). The samples with a positive reaction were confirmed by reverse-transcription polymerase chain reaction (RT-PCR). The results confirmed the presence of CMV, with an incidence of 2.8% in the study region. For the molecular characterization of the virus, a partial region of the RNA-dependent RNA polymerase (RdRp) gene was amplified and sequenced. The sequence analysis showed that the isolates shared nucleotide identities greater than 97.7% with GenBank accession MG696855.1, corresponding to a CMV isolate obtained from lulo (Solanum quitoense) in Colombia. Phylogenetic analysis grouped the sequences generated in this study with reference sequences from subgroup IA. Our results contribute to the understanding and updating of CMV population dynamics in this important plantain production region.

Keywords : CMV; ELISA; molecular detection; Musa AAB.

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