Abstract

ARANGO-MUNERA MV, Julián D. et al. Canine semen (Canis familiaris) cryopreservation on french straw in Medellín, Antioquia. rev. colombiana cienc. anim. Recia [online]. 2020, vol.12, n.1, pp.50-57.  Epub Aug 07, 2021. ISSN 2027-4297.  https://doi.org/10.24188/recia.v12.n1.2020.754.

The objective of this work was to apply the canine semen cryopreservation methodology in French straw in Medellín-Antioquia. A seminal collection was carried out to eight adult canines, by gloved hand method, each ejaculate was assessed macroscopically and microscopically and subsequently diluted with Triladyl® with a final concentration of 80x106 sperm/ ml. Semen samples were packed in 0.5 ml straws and a quick-freezing process was performed (nitrogen vapors for 25 minutes and then transferred directly to liquid nitrogen, french straw method). In fresh semen, a milky whitish appearance, a volume of 2.27 ml (± 1.40), a concentration of 388.5x106 sperm/mL (± 228.069), individual motility of 79% (± 4%) and vigor of 3.96 (± 0.327) were observed. 123 straws were obtained, of which 27 were taken randomly and individual motility (51 ± 19%) and vigor (2.89 ± 1.02) were evaluated. In conclusion, the application of the methodology of quick-freezing by french straw, using Triladyl® as a cryoprotectant, is suggested as an efficient protocol for cryopreservation of canine semen in Medellín-Antioquia.

Keywords : Biotechnology; dog; quick-freezing; reproduction.

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