SEX-DETERMINATION SYSTEMS AND THEIR EVOLUTION: MAMMALS

Sistemas de determinación de sexo y su evolución: caso mamíferos

FREDY A. COLORADO GARZÓN¹, M.Sc.; NUBIA E. MATTA CAMACHO¹, Ph. D.; ANTONIO SÁNCHEZ², Ph. D.

¹ Facultad de Ciencias, Departamento de Biología, Universidad Nacional de Colombia, Sede Bogotá, facoloradog@unal.edu.co

² Departamento de Biología Experimental, Universidad de Jaen, España

Presentado 11 de febrero de 2011, aceptado 6 de junio de 2011, correcciones 4 de noviembre de 2011.

ABSTRACT

Sex-determination methods are very diverse as they have become an enduring research field. Understanding the causes of gonadal development and elucidating the main factors involved in sex-determination of offspring required relating information from far-ranging areas such as cytology, embryology, morphology, molecular biology and even ecology and evolution. This article presents an overview of sex-determination in placental mammals, encompassing several levels of biological organization. The importance of the underlying molecular tools in the context of sex-determination assays and their implications in conservation genetics is also discussed.

Key words: Sex chromosomes, SRY-HMG, sexual reversion, genetic conservation and sex-determination.

RESUMEN

Los sistemas de determinación de sexo son muy diversos y en consecuencia se ha aumentado la investigación en este campo. Entender el desarrollo gonadal y elucidar los factores involucrados en la determinación de sexo de la descendencia ha requerido tomar información de aéreas como: citología, embriología, morfología, biología molecular e incluso de ecología y evolución. Este artículo presenta una visión de los mecanismos de determinación de sexo en mamíferos placentarios, abarcando algunos niveles de organización biológica. También se discute la importancia de las herramientas moleculares en las pruebas de determinación del sexo y sus implicaciones en conservación genética.

Palabras clave: cromosomas sexuales, SRY-HMG, reversión sexual, conservación genética y determinación de sexo.

Abbreviations:

SRY: Sex-determination Y chromosome region

ORF: Open Reading Frame

SOX: SRY-related gene

TDF: Testis Determining Factor

Abreviaciones:

SRY: Región determinante de sexo en el cromosoma Y ORF: Marco abierto de lectura

SOX: Genes relacionados al SRY

TDF: Factor determinante de testículo

INTRODUCTION

A fundamental process in most species concerns the sexual phenotype, which is being determined in future individuals during the embryo period. Thus, studying sexdetermination extends our knowledge about the genes and mechanisms involved in such a decision between two alternate embryonic routes: male or female phenotype.

The first attempts to understand sex-determination in mammals were made by removing the gonadal primordium in rabbit fetuses, resulting in all cases developing as females and therefore implying that the testicles were responsible for a male decision being made. Hence, it was established that differentiation into ovaries or testicles from the gonadal primordium determined the appearance of gender-specific structures during early development stages (Jost, 1953).

Subsequent research focused on finding a correlation between an individual's gender and their chromosomes, taking the case of Drosophila melanogaster as reference, where sex-determination depends on the relationship between the number of X chromosomes regarding autosomes, while the Y chromosome only carries ribosomal and fertility genes (Bernard, 1988; Hodgkin, 1990). However, studies in humans having chromosomal abnormalities (the so-called Turner and Klinefelter syndromes) have demonstrated the relevance of the Y chromosome in sex-determination in mammals (Ford et al., 1959; Goodfellow and Lovell-Badge, 1993).

SEX-DETERMINATION SYSTEMS DIVERSITY

Sexual differentiation has proven to be a very complex process, as are all others in individual development. Although sex-determination is of vital importance in vertebrate reproduction, the great variety of sex-determination mechanisms across different taxa is surprising. They can be divided into two broad categories during the course of evolution: external environment-dependent or environmental mechanisms (also known as: Environmental-dependent Sex Determination or ESD) and genetic mechanisms (Genetic Sex Determination or GSD). The developing embryo's sex is determined by an initial signal provided by the environment in the former while the offspring's sex depends on primary genetic signals in the latter (Bull, 1983; Janzen and Paukstis, 1991).

Sexual differentiation occurs in ESD in response to external signals after fertilization, so that the sex into which zygotes finally differentiate is independent of their genetic/ chromosomal composition. Many environmental factors are able to affect sex, although Temperature-dependent Sex Determination (TSD) is usually the most common form of environmental sex-determination (Korpelainen, 1990). This type of mechanism has been described in invertebrates, fish (Strüssmann and Patiño, 1995), and mainly in reptiles and some species of turtles and lizards (Ferguson and Joanen, 1982; Smith and Joss, 1993; Johnston et al., 1995; Pieau, 1996; Crews, 1996).

In organisms having genetic sex-determination, sex is "decided" at the moment of fertilization, depending on the gametes' chromosomal constitution. This does not necessarily mean that sex chromosomes should be heteromorphic, as evidenced by the fact that different degrees of differentiation can be shown, ranging from homomorphic sex chromosomes, to clear heteromorphism. The genes involved in sex-determination are thus present in a pair of chromosomes in some species that are indistinguishable under the microscope, while others are located on one of the heteromorphic chromosomes forming the sexual pair. In the latter case, heterogametic sex may be male (XX (♀) / XY (♂) systems), as in most mammals, or female (ZW (♀) / ZZ (♂) systems), as seen in birds and reptiles. The sex chromosomes may be highly conserved, as in the case of birds and mammals, or highly variable, even within species or genera, as in the case of amphibians (Wallis et al., 2008).

The variability observed in the two categories of sex-determination mechanisms goes beyond what one would expect for so widespread a process, given that even for organisms in which sex is determined in a similar way, the genetic mechanisms by which sexual determination and differentiation routes are implemented vary widely. This is the case of sex-determination mechanisms in the three best-studied model organisms: the fruit fly Drosophila melanogaster, the nematode Caenorhabditis elegans and the mouse Mus musculus (McElreavey et al., 1993; Ryner and Swain, 1995). Sex determination in Caenorhabditis and Drosophila in which the sex-determination signal depends on the relationship between the autosome and X chromosome dose is thus not comparable to what occurs in mice and most mammals where the presence of the Y chromosome leads to male development (Wallis et al., 2008). Likewise, sex-determination in mammals is made by the sex chromosome system XX (female)/XY (male), in which the presence of the Y chromosome is the dominant factor inducing male phenotype development. This pair of heteromorphic sex chromosomes originate from changes at evolutionary level, resulting from constant deletions and modifications which have led from originally homologous chromosomes to two distinct enough chromosomes X and Y differing in both morphology and gene content (Charlesworth, 1996). Such differentiation has been accompanied by the restriction or suppression of recombination between large regions of both chromosomes. Moreover, the Y chromosome has become drastically reduced in size, currently undergoing degeneration to become a generally small, gene-content poor, full of repeat sequences and mostly heterochromatic chromosome (Graves, 2006).

Thus, the presence of a Y chromosome in most mammals leads to morphological and hormonal changes which could be common to most species. One of the most significant changes is the development of testicles, although male gonads are indistinguishable from female organs during the early days of gestation. In fact, differentiation becomes morphologically visible in humans only six weeks after fertilization, while occurs 12.5 days post coitus (DPC) in mice (Koopman et al., 1990; Lovell-Badge and Hacker, 1995). Masculinization in mammals can consequently be understood as an alteration of gonadal development which, by default, might have led to producing ovaries (LovellBadge and Hacker, 1995).

The presence of testicles gives rise to the production of two hormones, namely antiMullerian hormone (AMH) (or Mullerian inhibiting substance MIS) and testosterone. The former is secreted by Sertoli cells and is responsible for degrading female structures which would eventually lead to the formation of the uterus and other female ducts. On the other hand, testosterone is produced by Leydig cells and is responsible for producing male ducts and external genitalia (Jost, 1953).

Exceptions to the importance of the Y chromosome in sex-determination in mammals have been observed in rodents, particularly in the wood lemming (Myopus schisticolor). Although the determination system is XX-XY (females and males, respectively), female XY can be found in this species in which, despite carrying the SRY gene (Y chromosome sexdetermining region, a determinant of masculinization in most mammals), their X chromosome can mask its effect (Fredga, 1994). Species have been described which lack the Y chromosome in their genome. For example, species such as Ellobius lutescens and Ellobius tancrei lack this chromosome, so that both males and females have the same karyotype: XO in E. lutescens and XX in E. tancrei (Fredga and Lyapunova, 1991; Just et al., 2002). Males from both species are fully fertile and have fully developed testicles. Despite the fact that the mammalian sex-determining gene, SRY, is also absent in these species, which indicates that could present a novel sex-determining mechanism that is independent of SRY and probably controlled by other master gene of the sex determination activation pathway. Recently, Kuroiwa et al., 2011, have demonstrated that in males of T. osimensis and T. tokunoshimensis there are two or three more copies of CBX2 per haploid genome than in females suggesting that these additional copies might be involved in the novel sexdetermining mechanism in species that lack SRY. However, although important effort is made to identify the control candidate gene in these species for the moment sex determination mechanism in absence of SRY and Y chromosome is a great enigma.

SEX CHROMOSOMES AND THEIR EVOLUTION

Current thinking considers that reptiles, birds and mammals' sex chromosomes were independently originated from different autosomes in their common ancestor, given their lack of homology (Fridolfsson et al., 1998). Part of such hypothesis has been confirmed by genetically mapping mammalian chromosomes XY and birds' ZW where there is no relationship between the members of the pairs (Nanda et al., 1999). However, these studies have revealed that mammalian X chromosome exhibits some homology with birds' different autosome regions, suggesting that sex chromosomes evolved from a pair of autosomes which are likewise conserved in other vertebrates.

It has therefore been assumed that the X and Y chromosomes in mammals evolved from an ancestral autosomal pair about 300 million years ago (Ohno, 1967; Graves and Shetty, 2001). It is believed that this evolutionary process began when a locus playing a dominant role in determining sex (the SRY gene) appeared in one of the members of the ancestral chromosome pair (the proto-Y chromosome). Degeneration began from the proto-Y chromosome which has led to it becoming the small, heterochromatic and barely gene populated Y chromosome currently common in most mammals. Such degeneration has taken place as a result of deleterious mutation accumulation in the non-recombinant region of chromosome Y. Furthermore, suppressing recombination would have been favored by the evolutionary process in grouping together and transmitting genes having male-specific functions (Graves and Shetty, 2001). Contrasting with degeneration, sex chromosomes have also undergone increases in size during their evolution by autosomal regions becoming added. Thus, comparative studies between Eutheria mammals, marsupials and monotremes have shown that a wide region of Eutheria X and Y chromosomes exhibit autosomal location in marsupials and monotremes. This region, which was originally autosomal, has been transferred by translocation to the sex chromosomes in ancestral species of Eutheria (Graves, 1995a; Glas et al., 1999). Another mechanism by which this chromosome might also have increased in size was repeat sequence amplification which would have accumulated on the Y chromosome during evolution (Graves, 2000).

Y chromosome degeneration, together with specific sequence amplification, resulted in this chromosome retaining only a small region of homology with the X chromosome, called the pseudoautosomal region (PAR; Graves, 1995b). Addition and degeneration are independent in each species or group of species and thus this region is neither the same nor has the same sequence in the sex chromosomes of all mammalian species, even when they are very close. However, this small homologous region is present in most mammalian sex chromosomes and is considered essential for allowing appropriate sex chromosome segregation during meiosis (Burgoyne, 1982; Ellis and Goodfellow, 1989). PARs are thus sex chromosome fragments which behave in an autosomal manner during meiotic crossover (Ellis and Goodfellow, 1989; Mohandas et al., 1992; Freije et al., 1992). XY sex chromosomes are found in the three subclasses of mammals (monotremes, marsupials and placentals) but there are significant differences in their interactions during meiosis and their numbers. Male platypus (Ornithorhynchus anatinus) have five pairs of sex chromosomes (Rens et al., 2004) and their recombination during meiosis (as in placental) is possible due to the PAR (Grützner et al., 2004). This fact contrasts with these chromosomes' recombination inability in marsupials lacking homologous regions (Graves and Watson, 1991).

The X chromosome in placentaries is essentially euchromatic and usually represents 5% of each species' haploid genome (Ohno, 1967). This chromosome contains about 1,500 genes (many being constitutively expressed) contained in 165Mb (Ross et al., 2005). The female produces random inactivation of X chromosomes in every cell to ensure equal doses of X-linked genes in males and females during embryonic development (Lyon, 1961). X chromosome size and gene content is highly conserved among different species, possibly ensuring correct functioning of the X chromosome inactivation mechanism (Ohno, 1967). It also has unique regions in monotremes and marsupials belonging to autosomes. In fact, it has been shown that autosomal translocation occurred after these groups' divergence (Graves, 1995a).

Placentaries' Y chromosome is small (60Mb), representing only 2% of the human genome. It contains a few genes (50 or so), most specializing in male functions, such as testicular determination and spermatogenesis (Graves, 2000; Graves, 2001; Skaletsky et al., 2003). For the most part, the Y chromosome consists of heterochromatic repeat sequences (Graves, 2000; Graves, 2001); moreover, these repeat sequences are poorly conserved among Y chromosomes from different species, even if they are evolutionarily closely-related. Like the X chromosome, Y shares regions with other mammal species whilst others are unique (Waters et al., 2001).

THE TDF GENE AND ITS RELATIONSHIP WITH SRY-HMG: IN SEARCH OF THE GENE DETERMINING MASCULINITY

Although the position of the testis determining factor (TDF) gene locus on human and mouse Y chromosome was already known in the 1980s, its cloning was not easy or as quickly as supposed at first. Throughout the history of the search for the testicle determining gene, several genes have once been considered equivalent to the TDF gene but have eventually been discarded due to evidence demonstrating their lack of involvement in testicular determination. Among these, the minor histocompatibility Y antigen (HY antigen) was one of the first candidates (Wachtel et al., 1975), followed by satellite DNA sequences known as banded krait minor satellite DNA (Bkm sequences) isolated from the W chromosome of the snake Bungarus fasciatus (Family Elapidae; Singh et al., 1976; Singh et al.,1979), and the zinc finger gene on the Y (ZFY) gene (Koopman et al., 1989). Final cloning of TDF was not possible until a detailed search was made of the minimum portion of the Y chromosome confering a male phenotype in humans became available. Thus, isolating specific regions of the Y chromosome (short arm distal), common in patients with testicular development and sex reversal (XX males), led to discovering an open reading frame (ORF) corresponding to the gene responsible for masculinity, known as the sex determining region of the Y chromosome (SRY). This gene has been confirmed as testicular determinant in subsequent research with mice (Sinclair et al., 1990; Gubbay et al., 1990; Page et al., 1990).

It is currently assumed that activating the gonadal differentiation pathway is initially controlled by the SRY gene, although this does not exclude the possibility that other genes having loci on the Y chromosome, X or even autosomes are involved in regulating its expression (Goodfellow and Lovell-Badge, 1993; Lovell-Badge and Hacker, 1995). The Sry transcript location in the embryonic genital ridge and adults' testicles supports its role as a testicle determinant (Koopman et al., 1990; Payen et al., 1996; Daneau et al., 1996). The SRY gene is usually single copy in most placental species and some marsupials although, in some species, it has multiple copies, some of them found on the X chromosome (Bianchi et al., 1993; Negamine, 1994; Bullejos et al., 1997). However, due to the presence of multiple internal stop codons, it is considered that some of these copies are actually pseudogenes (Marchal et al., 2008). Regarding the multiple copies found on the Y chromosome, they can be monoor polymorphic, as in the case of some species from the family Microtidae, although it remains unknown whether these copies are tandemly repeated or dispersed in the chromosome (Bullejos et al., 1999; Acosta et al., 2010). According to the rapid evolution of sex-determination systems hypothesis, research in some species of Ellobius (moles) and the spiny rat Tokudaia have confirmed that SRY is no longer responsible for determining masculinity, as it does not occur in the Y chromosome (the karyotypes for males and females being the same, XO in E. lutescens and T. osimensis and XX in E. tancrei; Just et al., 1995; Soullier et al., 1998). It is possible that even though there is a Y chromosome it may lack the SRY gene, as has been described in Microtus mandarinus mandarinus where the difference between males and females lies in X chromosome morphology (Chen et al., 2008).

The SRY gene is highly variable among species regarding both chromosomal location and nucleotide structure. Regarding its location, this locus is most proximal to the centromere of the Y chromosome's short arm in the mouse, while it is found in the most distal short arm in humans, precisely 5kb from PAR (Sinclair et al., 1990). This fact explains the high frequency of sex reversal cases, given that the SRY gene may be transferred to the X-chromosome due to errors in meiosis (Guellaen et al., 1984; Page, 1986; Capel et al., 1993). Concerning the SRY gene's nucleotide sequence, the mouse's has a very unusual structure because it is composed of a single 2,739 bp region having a long ORF without introns which is flanked by two nearly identical inverted repeats of at least 17 kb (Gubbay et al., 1992). This inverted repeat is absent in the human SRY gene (Sinclair et al., 1990; Lovell-Badge and Hacker, 1995).

Despite the foregoing variations, all SRY genes identified to date are genes without introns and have a highly conserved region in most mammals, called the high mobility group (HMG) box which encodes a 79 aminoacid long protein domain, characteristic of proteins whose activity is DNA binding (i.e. the SOX protein). This domain confers the property of producing folds in DNA on proteins acting as transcription factors (Ner, 1992; Pontiggia et al., 1994). Currently, there are no doubts about the importance of this HMG domain for proper SRY protein function, which has been confirmed in cases of sex reversal in humans resulting from mutations and small deletions in this domain (Goodfellow and Lovell-Badge, 1993).

However, the rest of the gene (regions flanking the HMG box) is highly variable in both nucleotide sequence and size, even among closely related species, thereby hampering their study. Thus, very little homology is shown when comparing nucleotide and aminoacid sequences outside humans, rabbits, mice and marsupials' SRY-HMG box. It has been proposed that these SRY sequences evolve faster because they present little functional significance, so that there are no functional restrictions preventing the accumulation of changes in sequence, so they are not subject to selective pressure (Whitfield et al., 1993; Tucker and Ludrigan, 1993).

Most research related to SRY has usually been directed towards studying its role in sex-determination at molecular level. Interestingly, it has also been characterized and used for sex-determination in various species such as gorilla, gazelle, rat, sheep, pig, mouse (Margarit et al., 1998), micro and mega bats (Bullejos et al., 2000), humans (Drobnic, 2006), canines and felines (Meyers, 2006), cattle (Lu et al., 2007) and goats (Shi et al., 2008). Furthermore, due to the high rate of molecular change, the accumulation of nonsynonymous substitutions and the absence of recombination (Whitfield et al., 1993; Tucker and Lundrigan, 1993), the SRY gene has been used as phylogenetic marker. Different authors have established phylogenetic relationships among species and genera within families of primates (Pamilo and O'Neill, 1997; Wang et al., 2002; Moreira, 2002), bovines (Payen and Cotinot, 1994) or felines (King et al., 2007).

THE SOX GENE FAMILY (SRY-RELATED, HMG BOX-CONTAINING GENES)

The SRY gene belongs to the SOX gene family consisting of genes whose proteins have greater than 60% identity in the HMG box regarding SRY (Goodfellow and Lovell-Badge, 1993). The DNA binding property afforded by that domain not only allows it to act as a transcription factor, but also as an architectural component of chromatin because it affects genetic material packaging (Canning and Lovell Badge, 2002). However, members of these proteins are not restricted to mammals and have been found in other groups such as insects, birds and reptiles (Griffiths, 1991; Foster et al., 1992).

There are more than 20 known SOX genes in mammals and, although they have functions related to various aspects of embryogenesis, most of them are not involved in sex determination (Prior and Walter, 1996; Pevny and Lovell-Badge, 1997). SOX3 is a member of this gene family; its locus is located in the X chromosome (Stevanovic et al., 1993). There is evidence supporting the origin of the SRY gene from a primitive SOX gene present in the ancestral evolutionary line before the separation of Metatheria and Eutheria. The presence of the SOX3 gene in the X chromosome of all mammals tested and the high degree of homology between the HMG box of the SRY genes and the chromosome suggests parallel evolution for these genes from an ancestral autosomal pair that gave rise to the sex chromosomes (Stevanovic et al., 1993; Foster and Graves, 1994; Collignon et al., 1996).

On the other hand, the SOX9 gene is involved in sex determination (Foster et al., 1992; Wagner et al., 1994; Collingnon et al., 1996). The SOX9 gene is highly conserved among fish, birds and mammals and its expression is specific to the male gonads of birds and mammals, for example 11.5 DPC in mice, with no expression in females. This is why it is considered to be a gene which is involved in the formation of male gonads and may be regulated by the SRY gene (Morais et al., 1996).

A finding related to the importance of SOX9 in sex determination has been a case of sex reversal in humans (XX) resulting from this gene's duplication on chromosome 17 which led to the development of testes (Huang et al., 1999). This fact corroborates SOX9's potential (if expression threshold-dependent) to become a candidate gene for allowing the development of masculinity in the absence of SRY, mimicking the latter's function. This may have been due to the high degree of identity between HMG boxes from SRY and SOX9 (76% at protein level), explaining why chimeric SRY genes (having SOX9 boxes) have allowed masculinization of transgenic mice (Bergstrom et al., 2000). Although little information is available about SRY-controlled genes, it has been suggested that SOX9 is usually controlled by SRY (Canning and Lovell Badge, 2002).

When comparing the HMG box of distantly related species, the high identity between them becomes evident (Coriat et al., 1993). However, the SRY-HMG box has specific and critical amino acids for its function to differentiate it from the HMG box of SOX genes (Gubbay et al., 1990; Berta et al., 1990; Coriat et al., 1993).

MOLECULAR SEXING AND GENETIC CONSERVATION

Sex identification is of great importance in evolutionary biology, ecology, prenatal diagnosis, forensic identification, population genetics, conservation and planning studies concerning the reproduction of threatened or clearly endangered species (Fridolfsson and Ellegren, 1999; Griffiths and Phil, 2000; Drobnic, 2006; Matta et al., 2009). Sexing using molecular tools has been particularly important for testing and controlling poaching in which the capture and killing rate should be differential between males and females in bird breeding programs where sexual dimorphism is not obvious. It also represents an alternative to sexing methodologies such as laparotomy or laparoscopy (Fridolfsson and Ellegren, 1999; Griffiths and Phil, 2000; Matta et al., 2009; North American Wildlife Enforcement Group-NAWEG-2000); research in its field is also relevant in conservation biology, ecology and wildlife management.

There is an increasing interest in the use of non-invasive sampling for the study of wild animal populations to avoid disturbance of the animals and to get results with indirect and easy sampling, such as skin, hair, feathers, nails, feces, or simple drops of blood. Identification of the individual's sex is important for conservation studies of wild animals. However in some species, limitations regarding sex determination using molecular tools are associated with the problem of finding the specific sequences in sex chromosomes that differentiate between males and females and can be demonstrated by methodologies such as PCR amplification or differential cleavage with restriction enzymes. The gene does not necessarily have to involve pathways leading to sexual differentiation. Other genes or even non-encoding sequences may be responsible for other functions, although they must have differences between the sex chromosomes that are not in autosomes.

Several molecular methods for sexing can easily be adapted for a wide range of species. In mammals, RFLP analysis based on Xand Ychromosome-specific polymorphism has been extensively used, as for example those observed in the AMELX⁄AMELY and ZFX/ZFY genes, located either on the X and Y chromosome respectively (Villesen and Fredsted, 2006; Lindsay and Belant, 2008; Xu et al., 2008; Li et al., 2010). However, in some species some sexing methods encounter difficulties in the amplification of long fragments from degraded DNA and in non-specific amplification from sample analysis. However, PCR products of considerable length are used, which is not suitable for degraded DNA such as faeces analysis. Hence the alternative approach consisted in the amplification of short Y-specific genes using conserved primers, as for example the SRY (Gupta et al., 2006) and Ube1Y genes (Sloane et al., 2000).

However, the use of Y-chromosome specific sequences alone is not informative because an absence of PCR product can indicate either a female or a failed reaction. Hence, these reactions are often performed in conjunction with another fragment to act as a positive control for amplification, such as a mitochondrial DNA or a nuclear locus, being preferable the nuclear one as mitochondrial DNA are present in more copy number than nuclear DNA and hence than the amplified Y chromosome specific markers.

Some limitations and considerations should be taken into account in molecular genetic analysis when the genome of the species of interest is scarcely studied and we are searching for target genes: 1) finding species-related genes (even if they are orthologous) does not necessarily involve a similar task, considering that the biological contexts of expression can be very different; 2) the genes of interest could not be located on the same chromosomes; 3) the gene sequences could not be conserved enough to permit to amplify with interspecific primers or cut with the same restriction endonucleases; 4) regarding the function itself, there is still a lot of uncertainty in terms of metabolic pathway flexibility when induced mutations might not be apparent in the phenotype as a result of possible substitutions by other proteins. These guidelines should be kept in mind when exploring little-studied species using databases and to avoid the pitfall of biological reductionism (Marin and Baker, 1998).

CONCLUSIONS

Studying sex determination is of wide interest due to the great variability of systems present even among species from the same gender, contrary to what has previously been thought, i.e. that the relationship between XX/XY karyotypes and individuals' phenotype does not have to be so linear and, even less so, is always not only dependent on specific chromosomes but also on the genes and alleles responsible for masculinity.

DNA-based methodologies for identifying sex are valuable tools due to their speed, sensitivity and the possibility of easy sampling of meat, skin, feathers, nails, or simple drops of blood, thereby minimizing pain and stress during sampling. Moreover, they can be performed on very small tissue samples obtained from hunting-control programs. Correct sex determination is important when studying a population's behavior and social structure and for conservation plans where decisions must be taken by adopting an interdisciplinary approach.

ACKNOWLEDGEMENTS

The authors are grateful for the financial support provided by the Universidad Nacional de Colombia's Bogota Research Division, Project No 8003270, the Orinocences Studies Institute, Orinoco Campus, project No 10307. This work was also supported by the Spanish 'Ministerio de Ciencia y Tecnología' through project number CGL2009-07754 (cofounded by the European Regional Development Fund, FEDER) and by Junta de Andalucía throughout their program 'Ayudas a grupos de investigación' (CVI 220).

BIBLIOGRAPHY

ACOSTA M, MARCHAL J, ROMERO-FERNÁNDEZ I, MEGÍAS-NOGALES B, MODI W, SÁNCHEZ BACA A. Sequence analysis and mapping of the SRY gene in species of the subfamily Arvicolinae (rodentia). Sex Dev. 2010;4:336-347.        [ Links ]

BERTA P, HAWKINS A, SINCLAIR A, TAYLOR B, GRIFFITHS P, GOODFELLOW P, et al. Genetic evidence equating SRY and the testis determining factor. Nature.1990;348:448-450.        [ Links ]

BERNARD J. The biology of heterochromatin. In: Verma R, editor. Heterochromatin molecular and structural aspects. Cambridge University; 1988. p. 1-147.        [ Links ]

BERGSTROM D, YOUNG M, KENNETH H, ALBRECHT E, EICHER E. Related function of mouse SOX3, SOX9 and SRY HMG domains assayed by male sex determination. Genesis. 2000;28:111-124.        [ Links ]

BIANCHI N, BIANCHI M, BAILLIET G, DE LA CHAPELLE A. Characterization and sequencing of the sex determining region Y gene (SRY) in akodon (Cricetidae) sp. Chromosoma. 1993;102:389-395.        [ Links ]

BULLEJOS M, SÁNCHEZ A, BURGOS M, HERA C, JIMÉNEZ R, DÍAZ DE LA GUARDIA R. Multiple, polymorphic copies of SRY in both males and females of the vole Microtus cubrerae. Cytogenet Cell Genet. 1997;79:167-171.        [ Links ]

BULLEJOS M, SÁNCHEZ A, BURGOS M, JIMÉNEZ R, DÍAZ DE LA GUARDIA R. Multiple mono-and polymorphic Y-linked copies of the SRY HMG-box in Microtidae. Cytogenet Cell Genet. 1999;86:46-50.        [ Links ]

BULLEJOS M, SÁNCHEZ A, BURGOS M, JIMÉNEZ R, DÍAZ DE LA GUARDIA R. The SRY gene HMG-box in microand megabats. Cytogenet Cell Genet. 2000;88:30-34.        [ Links ]

BULL J. In: "The evolution of sex determining mechanisms". Benjamin/Cummings. Menlo Park, California; 1983.        [ Links ]

BURGOYNE PS. Genetic similarity and crossing over in the X and Y chromosomes of humans. Hum Genet. 1982;61:85-90.        [ Links ]

CAPEL B, RASBERRY C, DYSON J, BISHOP C, SIMPSON E, VIVIAN N, et al. Deletion of Y chromosome sequences located outside the testis determining region can cause XY female sex reversal. Nature Genet. 1993;5:301-307.        [ Links ]

CHARLESWORTH B. The evolution of chromosomal sex determination and dosage compensation. Curr Biol. 1996;6:149-162.        [ Links ]

CHEN Y, DONG Y, XIANG X, XINRUI E, ZHU B. Sex determination of Microtus mandarinus mandarinus is independent of SRY gene. Mamm Genome. 2008;19, 61-68.        [ Links ]

CANNING C, LOVELL-BADGE R. SRY and sex determination: How lazy can it be?. Trends Genet. 2002; 18:111-112.        [ Links ]

COLLIGNON J, SOCKANATHAN S, HACKER A, COHENTANNOUDJI M, NORRIS D, RASTAN S, et al. A comparison of the properties of SOX-3 with SRY and two related genes, SOX-1 and SOX-2. Development. 1996;122:509-527.        [ Links ]

CORIAT A, MÜLLER U, HARRY J, UWANOGHO D, SHARPE P. PCR amplification of SRY-related gene sequences reveals evolutionary conservation of the SRY-box motif. Genome Res. 1993;2:218-222.        [ Links ]

CREWS D. Temperature-dependent sex determination: the interplay of speroid hormones and temperature. Zool Sci. 1996;13:1-13.        [ Links ]

DANEAU I, ETHIER J, USSIER L, SILVERSIDES D. Porcine SRY locus and genital ridge expression. Biol Reprod. 1996;55:47-53.        [ Links ]

DROBNIC K. A new primer set in a SRY gene for sex identification. International Congress Series. 2006;1288:268-270.        [ Links ]

ELLIS N, GOODFELLOW P. The mammalian pseudoautosomal region. Trends Genet. 1989;5:406-410        [ Links ]

FERGUSON M, JOANEN T. Temperature egg incubation determines sex in Alligator mississippiensis. Nature. 1982;296:850-853.        [ Links ]

FORD C, JONES K, POLANI P, DE ALMEIDA J, BRIGGS J. A sex chromosome anomaly in a case of a gonadal dysgenesis (Turner's syndrome). Lancet. 1959;1:711-713.        [ Links ]

FOSTER J, BRENNAN F, HAMPIKIAN G, GOODFELLOW P, SINCLAIR A, LOVELL-BADGE R, et al. Evolution of sex determination and the Y chromosome: SRY related sequences in marsupials. Nature. 1992;359:531-533.        [ Links ]

FOSTER J, GRAVES J. An SRY-related sequence on the marsupial X chromosome: implications for the evolution of the mammalian testis determining gene. Proc Natl Acad Sci U S A. 1994;91:1927-1931.        [ Links ]

FREDGA K. The differences between the sexes. R V short and E, Balaban editors. Cambridge University; 1994. p. 419-431.        [ Links ]

FREDGA K, LYAPUNOVA E. fertile males with two X chromosomes in Ellobius tancrei (Rodentia, Mammalia). Hereditas. 1991;115:86-97.        [ Links ]

FREIJE D, HELMS C, WATSON S, DONISKELLER H. Identification of a second pseudoautosomal region near the Xq and Yq telomeres. Science. 1992;258:1784-1787.        [ Links ]

FRIDOLFSSON A, CHENG H, COPELAND G, JENKINS N, CHING L, RAUDSEPP T, et al. Evolution of the avian sex chromosomes from an ancestral pair of autosomes. Proc Natl Acad Sci U S A. 1998;95:8147-8152.        [ Links ]

FRIDOLFSSON A, ELLEGREN H. A simple and universal method for molecular sexing of non-ratite birds. J Avian Biol. 1999;30:116:121.        [ Links ]         [ Links ]

GOODFELLOW P, LOVELL-BADGE R. SRY and sex determination [summary]. Annu Rev Genet. 1993;27:71-92.        [ Links ]

GRAVES J. The origin and function of the mammalian Y chromosome and Yborne genes an evolving understanding. BioEssays. 1995a;17:311-320.        [ Links ]

GRAVES J. The evolution of mammalian sex chromosomes and the origin of sex determining. Phil. Trans. R. Soc. Lond. B. 1995b;350:305-312.        [ Links ]

GRAVES J. Human Y chromosome, sex determination, and spermatogenesisa feminist view. Bio. Reprod. 2000;63:667-676.        [ Links ]

GRAVES J. From brain determination to testis determination: evolution of the mammalian sex-determining gene. Reprod. Fertility. Dev. 2001;13:665-672.        [ Links ]

GRAVES J. Sex chromosome specialization and degeneration in mammals. Cell. 2006;124:901-914.        [ Links ]

GRAVES J, SHETTY S. Sex from W to Z: evolution of vertebrate sex chromosomes and sex determining genes. J. Exp. Zool. 2001;290:449-462.        [ Links ]

GRAVES J, WATSON J. Mammalian sex chromosomes: evolution of organization and function. Chromosoma. 1991;101:63-68.        [ Links ]

GRIFFITHS R. The isolation of conserved DNA sequences related to the human sex-determining region Y gene from the lesser black-backed gull (Larus fuscus). Proc R Soc London Biol. 1991;244:123-128.        [ Links ]

GRIFFITHS R, PHIL D. Sex identification in birds. Seminars in avian and exotic pet medicine. 2000;9:14-26.        [ Links ]

GRÜTZNER F, RENS W, TSEND-AYUSH E, EL-MOGHARBEL N, O'BRIEN P, JONES R, et al. In the platypus a meiotic chain of ten sex chromosomes shares genes with the bird Z and mammal X chromosomes. Nature. 2004;432: 913-917.        [ Links ]

GUBBAY J, COLLIGNON J, KOOPMAN P, CAPEL B, ECONOMOU A, MÜNSTERBERG A, et al. A gene mapping to the sex determining region of the mouse Y chromosome is a member of a novel family of embryonically expressed genes. Nature. 1990;346:245-250.        [ Links ]

GUBBAY J, VIVIAN N, ECONOMOU A, JACKSON D, GOODFELLOW P. Inverted repeat structure of the SRY locus in mice. Proc Natl Acad Sci U S A. 1992;89:7953-7957.        [ Links ]

GUELLAEN G, CASANOVA M, BISHOP C, GELDWORTH D, ANDRE G, FELLOUS M, et al. Human XX males with single copy DNA fragments. Nature. 1984;307:172-173.        [ Links ]

GUPTA S, THANGARAJ K, SINGH L. A simple and inexpensive molecular method for sexing and identification of the forensic samples of elephant origin. J Forensic Sci. 2006;51:805-807.        [ Links ]

HODGKIN J. Sex determination compared in Drosophila and Caenorhabditis. Nature. 1990;344:721-728.        [ Links ]

HUANG B, WANG S, SNING Y, LAMB A, BARTLEY J. Autosomal XX reversal caused by duplication of SOX9. Am J Med Genet. 1999;87:349-353.        [ Links ]

JANZEN F, PAUKSTIS G. Environmental sex determination in reptiles: ecology, evolution, and experimental design. Quart Rev Biol. 1991;66:149-179.         [ Links ]

JOHNSTON C, BARNETT M, SHARPE P. The molecular biology of temperaturedependent sex determination. Phil Trans R Soc Lond B. 1995;350:297-304.        [ Links ]

JOST A. Problems of fetal endocrinology; the gonadal and hypophyseal hormones. Rec prog. Horm. 1953;8:379-418.        [ Links ]

JUST W, BAUMSTARK A, HAMEISTER H, SCHREINER B, REISERT I, HAKHVERDYAN M, VOGEL W. The sex determination in Ellobius lutescens remains bizarre. Cytogenet Genome Res. 2002;96:146-153.        [ Links ]

JUST W, RAU W, VOGEL W, AKHVERDIAN M, FREDGA K, GRAVES J, et al. Absence of Sry in species of the vole Ellobius. Nature Genet. 1995;11:117-118.        [ Links ]

KING V, GOODFELLOW P, PEARKS A, JOHNSON W, O BRIEN S, PECONSLATTERY J. Evolution of the male-determining gene SRY within the cat family Felidae. Genetics. 2007;175:1855-1867.        [ Links ]

KOOPMAN P, GUBBAY J, COLLIGNON J, LOVELL-BADGE R. Zfy expresion patterns are not compatible with a primary role in mouse sex determination. Nature. 1989;348:450-452.        [ Links ]

KOOPMAN P, MÜNSTERBERG A, CAPEL B, VIVIAN N, LOVELL-BADGE R. Expression of a candidate sex determining gene during mouse testis differentiation. Nature. 1990;348:450-452.        [ Links ]

KORPELAINEN H. Sex ratios and conditions required for environmental sex determination in animals. Biol Rev. 1990;65:147-184.        [ Links ]

KUROIWA A, HANDA S, NISHIYAMA C, CHIBA E, YAMADA F, ABE S, et al. Additional copies of CBX2 in the genomes of males of mammals lacking SRY, the Amami spiny rat (Tokudaia osimensis) and the Tokunoshima spiny rat (Tokudaia tokunoshimensis). Chromosome Res. 2011;19:635-644.        [ Links ]

LI Y, XU X, ZHANG L, ZHANG Z, SHEN F, ZHANG W, YUE B. An ARMS-based technique for sex determination of red panda (Ailurus fulgens). Mol Ecol Resour. 2011;11:400-403.        [ Links ]

LINDSAY A, BELANT J. A simple and improved PCR-based techniquefor whitetailed deer (Odocoileus virginianus) sex identification. Conservation Genetics. 2008;9:443-447.        [ Links ]

LOVELL-BADGE R, HACKER A. The molecular genetics of SRY and its role in mammalian sex determination. Phil Trans R Soc Lond B. 1995;350:205-214.        [ Links ]

LU W, RAWLINGS N, ZHAO J, WANG H. Amplification and application of the HMG box of bovine SRY gene for sex determination. Anim. Reprod. Sci. 2007;186-191.         [ Links ]

LYON M. Gene action in the X-chromosome of the mouse (Mus musculus L.). Naturwissenschaften. 1961;190:372-373.        [ Links ]

MARCHAL J, ACOSTA M, BULLEJOS M, DÍAZ DE LA GUARDIA R, SÁNCHEZ A. Origin and spread of the SRY gene on the X and Y chromosomes of the rodent Microtus cabrerae: role of L1 elements. Genomics. 2008;91:142-151.        [ Links ]

MARGARIT E, GUILLÉN A, REBORDOSA C, VIDAL T, SÁNCHEZ M, BALLESTA F, et al. Identification of conserved potentially regulatory sequences of the SRY gene from 10 different species of mammals. Biochem. biophysBiophy. Res. Ccommun. 1998;245:370-377.        [ Links ]

MARIN I, BAKER B. The Evolutionary Dynamics of Sex Determination [summary]. Science. 1998;281:1990-1994.        [ Links ]

MATTA N, RAMÍREZ N, ZÚÑIGA B, VERA V. Determinación de sexo en aves mediante herramientas moleculares. Acta biol Colomb. 2009;14:25-38.        [ Links ]

MEYERS W. Genetics, genomics, and molecular biology of sex determination in small animals. Theriogenology. 2006;66:1655-1658.        [ Links ]

MCELREAVEY K, VILAIN E, COTINOT C, PAYEN E, FELLOWS M. Control of sex determination in animals. Eur J Biochem. 1993;218:769-783.        [ Links ]

MOHANDAS T, SPEED R, PASSAGE M, YEN P, CHANDLEY A, SAPHIRO L. Role of the pseudoautosomal region in sex-chromosome pairing during male meiosis: meiotic studies in a man with a deletion of distal Xp. Am J Hum Genet. 1992;51:526-533.        [ Links ]

MORAIS D, HACKER A, HARLEY V, GOODFELLOW P, SWAIN A, LOVELLBADGE R. Sox9 expression during gonadal development implies a conserved role for the gene in the testis differentiation in mammals and birds. Nat Genet. 1996;14: 62-68.        [ Links ]

MOREIRA M. Sry evolution in Cebidae (Platyrrhini: Primates). J Mol Evol. 2002;55:92-103.        [ Links ]

NANDA I. SHAN Z, SCHARTL M, BURT D, KOEHLER M, NOTHWANG H, et al. 300 million years of conserved synteny between chicken Z and human chromosome 9. Nat Genet. 1999;21:258-259.        [ Links ]

NAWEG North American Wildlife Enforcement GroupDNA Analysis in wildlife forensic, Information Bulletin (2000). http:77www.ec.gc.ca        [ Links ]

NEGAMINE C. The testis determining gene, SRY, exists in multiple copies in old world rodents. Genet Res. 1994;64:151-159.         [ Links ]

NER S. HMGs everywhere. Curr Biol. 1992;2:208-10.        [ Links ]

OHNO S. Sex Chromosomes and Sex-Linked Genes. Springer-Verlag; 1967. PAGE D. Sex reversal: deletion mapping the male-determining function of the human Y chromosome. In: Cold Spring Harbor symposium on quantitative biology, editors. New York, Molecular biology of Homo sapiens; 1986;51:229-235.        [ Links ]

PAGE D, FISHER E, McGILLIVRAY B, BROWN L. Additional deletion in sexdetermining region of human Y chromosome resolves paradox of X1t (Y,22) female. Nature. 1990;346:279-281.        [ Links ]

PAMILO P, O'NEILL R. Evolution of the Sry genes. Mol Biol Evol. 1997;14:49-55.         [ Links ]

PAYEN E, PAILHOUX E, MERHI R, GIANQUINTO L, KIRSZENBAUM M, LOCATELLI A, COTINOT C. Characterization of ovine SRY transcript and developmental expression of genes involved in sexual differentiation. Int J Dev Biol. 1996;40:567-575.        [ Links ]

PAYEN J, COTINOT C. Sequence evolution of SRY gene within Bovidae family. Mamm. Genome. 1994;5:723-725.        [ Links ]

PEVNY L, LOVELL-BADGE R. Sox genes find their feet. Curr Op Genet Dev. 1997;7:338-344.        [ Links ]

PIEAU C. Temperature variation and sex determination in reptiles. BioEssays. 1996;18:19-26.        [ Links ]

PONTIGGIA A, RIMINI R, HARLEY V, GOODFELLOW P, LOVELL-BADGE R, BIANCHI M. Sexreversing mutations affect the architecture of SRY-DNA complexes. EMBO J. 1994; 13:6115-6124.        [ Links ]

PRIOR H, WALTER M. SOX genes: architects of development. Mol Med. 1996;2:405-412.         [ Links ]

RENS W, GRÜTZNER F, O'BRIEN P FAIRCLOUGH H, GRAVES J, FERGUSONSMITH M. Resolution and evolution of the duck-billed platypus karyotype with an X1Y1X2Y2X3Y3X4Y4X5Y5 male sex chromosome constitution. Proc Natl Acad Sci U S A. 2004;101:16257-16261.        [ Links ]

ROSS M, GRAFHAM D, COFFEY A, SCHERER S, MCLAY K, MUZNY D, et al. The DNA sequence of the human X. Chromosome. Nature. 2005;434:325-337.        [ Links ]

RYNER L, SWAIN A. Sex in the '90s. Cell. 1995;81:483-493.        [ Links ]

SHI L, YUE W, REN Y, LEI F, ZHAO J. Sex determination in goat by amplification of the HMG box using duplex PCR. Anim. Reprod. Sci. 2008;105:398-403.        [ Links ]

SINCLAIR A, BERTA P, PALMER M, HAWKINS J, GRIFFITHS B, SMITH M, et al. A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif. Nature. 1990;336:780-783.        [ Links ]

SINGH L, PURDOM I, JONES K. Satellite DNA and evolution of sex chromosomes. Chromosoma. 1976;59:43-62.        [ Links ]

SINGH L, PURDOM I, JONES K. Behaviour of sex chromosome associated satellite DNAs in somatic and germ cells in snakes. Chromosoma. 1979;71:167-181.        [ Links ]

SKALETSKY H., KURODA-KAWAGUCHI T, MINX P, CORDUM H, HILLIER L, BROWN L, et al. The male-specific region of the human Y chromosome is a mosaic of discrete sequence classes. Nature. 2003;423:825-837.        [ Links ]         [ Links ]

SMITH C, JOSS J. Temperature-dependent gonadal differentiation in the alligator. In: "Sex chromosomes and sex-determining genes". Eds: K.C. Reed y J.A. Marshall Graves. Harwood Acadamic Publishers; 1993. p. 7-15.        [ Links ]

SOULLIER S, HANNI C, CATZEFLIS F, BERTA P, LAUDET V. Male sex determination in the spiny rat Tokudaia osimensis (Rodentia: Muridae) is not Sry dependent. Mamm. Genome. 1998;9:590-592.        [ Links ]

STEVANOVIC M, LOVELL-BADGE R, COLLIGNON J, GOODFELLOW P. SOX3 is an X-linked gene related to SRY. Hum Molec Genet. 1993;2:2007-2012.        [ Links ]

STRÜSSMANN C, PATIÑO R. Temperature manipulation of sex differentiation in fish. In: "Proceedings of the Fish International Symposium on the Reproductive Physiology of Fish". Eds: F. Goetz and P. Thomas. FishSymp95, Austin, Texas; 1995. p. 153-157.        [ Links ]

TUCKER P, LUNDRIGAN B. Rapid evolution of the sex determining locus in Old World mice and rats. Nature. 1993:364;715-717.        [ Links ]

VILLESEN P, FREDSTED T. A new sex identification tool: one primer pair can reliably sex ape and monkey DNA samples. Conserv. Genet. 2006;7:455-459.        [ Links ]

WACHTEL S, OHNO S, KOO G, BOYSE E. Possible role for H-Y antigen in the primary determination of sex. Nature. 1975;257:235-236.        [ Links ]

WAGNER T, WIRTH J, MEYER J, ZABEL B, HELD M, et al. Autosomal sex reversal and campomelic dysplasia are caused by mutations in and around the SRY related gene SOX9. Cell. 1994; 79:1111-1120.        [ Links ]

WANG X, ZHANG J, ZHANG Y. Erratic evolution of SRY in higher primates. Mol Biol Evol. 2002;19:582-584.        [ Links ]

WALLIS M, WATERS P, GRAVES J. Sex determination in mammals before and after the evolution of SRY. Cell Mol Life Sci. 2008;65:3182-3195.        [ Links ]

WATERS P, DUFFY B, FROST C, DELBRIDGE M, GRAVES J. The human Y chromosome derives largely from a single autosomal region added 80-130 million years ago. Cytogenet. Cell Genet. 2001;93:315-316.        [ Links ]

WHITFIELD L, LOVELL-BADGE R, GOODFELLOW P. Rapid sequence evolution of the sex determining gene SRY. Nature. 1993;364:713-715.        [ Links ]

XU X, LIN L, ZHANG Z, SHEN F, ZHANG L, YUE B. A reliable, non invasive PCR method for giant panda (Ailuropoda melanoleuca) sex identification. Conserv. Genet. 2008;9:739-741.         [ Links ]