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Caldasia

Print version ISSN 0366-5232

Caldasia vol.40 no.1 Bogotá Jan./June 2018

https://doi.org/10.15446/caldasia.v40n1.63654 

Notas breves

Domestic horses within the Maya biosphere reserve: A possible threat to the Central American tapir (Tapirus bairdii)

Caballos dentro de la reserva de la biosfera Maya: Una posible amenaza para el tapir Centroamericano (Tapirus bairdii)

MANUEL LEPE-LÓPEZ 1   * 

RONY GARCÍA-ANLEU 2  

NICHOLAS M. FOUNTAIN-JONES 3  

GABRIELA PONCE 2  

MARIANO GONZALEZ 2  

LUIS E. ESCOBAR 4  

1 PhD Program in Conservation Medicine, Facultad de Ciencias de la Vida, Universidad Andres Bello, República 252, Santiago, Chile.

2 Wildlife Conservation Society, Flores Island, Petén, Guatemala.

3 Department of Veterinary Population Medicine, University of Minnesota, Saint Paul, Minnesota, USA.

4 Department of Fish and Wildlife Conservation, Virginia Tech, Blacksburg, Virginia 24061, USA.


ABSTRACT

The Central American tapir (Tapirus bairdii) is the largest herbivore in the Neotropics classified as “endangered.” It has been proposed that Equine Infectious Anemia virus (EIA) is a disease of horses with potential to lead to further decline of T. bairdii populations. In this study, we used domestic horses as sentinels for EIA in the Maya Biosphere Reserve in Guatemala. In total, 40 % (13) horses tested were seropositive to EIA. This study may inform wildlife management strategies inside protected areas by considering the threat from incursions of domestic animals inside core areas of natural reserves.

Key words: Equine Infectious Anemia disease; Lentivirus; Spillover; Wildlife diseases

RESUMEN

El tapir centroamericano (Tapirus bairdii) es el herbívoro más grande del Neotrópico clasificado “en peligro de extinción”. Ha sido propuesto que la Anemia Infecciosa Equina (AIE) es una enfermedad de caballos con potencial de provocar una declinación de las poblaciones de T. bairdii. En este estudio utilizamos caballos domésticos como centinelas para AIE en la Reserva de la Biosfera Maya en Guatemala. En total, el 40 % (13) caballos evaluados fueron seropositivos a AIE. Este estudio puede orientar las estrategias de manejo de áreas protegidas, considerando la amenaza de incursiones de animales domésticos en zonas núcleo de reservas naturales.

Palabras clave: Enfermedad Anemia Infecciosa Equina; Lentivirus; Transmisión; Enfermedades de vida silvestre

Spillover of infectious diseases (i.e., interspecific transmission) from domestic animals to wildlife occurs often but is poorly documented (Power and Mitchell 2004). Nevertheless, these events are a threat to the conservation of species with reduced populations (Craft et al. 2009). Furthermore, the spillover of pathogens is more frequent between species genetically related.

The Central American tapir Tapirus bairdii Gill, 1865, is the largest terrestrial herbivore in the Neotropics, and is classified as “endangered” by the International Union for Conservation of Nature (IUCN) Red List; populations have declined ~50 % in the last thirty years (García et al. c2017). Infectious diseases, combined with illegal hunting and habitat loss, are a significant threat for tapir conservation (Pedersen et al. 2007). In Mexico, transmission of diseases from horses to T. bairdii have been reported, mainly helminths of the genus Trichostrongylus (Aldán et al. 2006).

Equine Infectious Anemia virus (EIA; Lentivirus of the Retroviridae family) has been considered a potential risk for tapirs (Mangini et al. 2012). EIA virus is a vector-borne disease transmitted principally by hematophagous tabanid flies (Diptera: Tabanidae; Issel and Foil 1984). However, in the Atlantic Forest and Pantanal Biomes of Brazil (1996-2012), especially in the Morro do Diabo Park, 100 % tapirs (Tapirus terrestris Linnaeus, 1758) were found to be negative to EIA virus antibodies (May Júnior 2011, Medici et al. 2014). The presence of horses in the Morro do Diabo Park, however, was not reported in this study, reducing the information necessary to analyze spillover events thoroughly.

The Maya Biosphere Reserve (MBR) is considered a Mesoamerican biodiversity hotspot and has the largest tapir population in Guatemala (Vettorazzi and Manrique 2016). Monzón and Schuster (2013) reported the presence of biting tabanid flies of the genera Tabanus and Chlorotabanus in the MBR. We attempted to answer a crucial question that may help clarify the risk to EIA infection for tapirs: Is there virus circulation in domestic horses in areas where tapirs are present?

We conducted a serosurveillance in December 2012 in the MBR. The tapir population has been estimated to be ~120 individuals in the ~1,170 km² reserve (García et al. c2009). Horses in the reserve are used for transportation during archaeological explorations and tourist transportation from the edges to core areas of the reserve, including to the archaeological sites El Mirador and Rio Azul where tapir are present (García et al. c2009). We looked for prior exposure to EIA virus in horses living inside the reserve in Carmelita Community, via identification of EIA antibodies in the blood serum.

We collected 5 ml of blood from horses to obtain the serum. Serological analysis (agar gel immunodiffusion; Coggins et al. 1972) was conducted at the Microbiology Laboratory of the Faculty of Veterinary Medicine, University of San Carlos, in Guatemala City.

Although Guatemalan law prohibits the entry of domestic animals to core areas of natural reserves (CONAP c2001), we observed that the use of horses in the nucleus of the MBR is a common practice. From the serological survey, thirteen samples (40 %) out of the 32 collected were positive for EIA, one horse was determined suspect, and 18 were negative to the agar gel immunodiffusion test. Strikingly, our serology results show that the horse population inside the reserve had previous exposure and possible infection to EIA, making these horses potential carriers of the EIA virus in the natural reserve. Under this scenario, risk of EIA spillover from horses to wild tapirs exists in the Guatemalan reserve. Seropositive horses were present in areas where plausible EIA vectors have been reported (Monzón and Schuster 2013).

Previous attempts to find evidence of EIA infection in wild tapir in other countries have been unsuccessful. For example, three studies attempting to identify antibodies against EIA in T. terrestris at three sites in Brazil had negative test results (Medici et al. 2014, Furtado et al. 2010, May Junior 2011). Thus, this virus has not been reported in individuals of the Tapirus genus to date (Medici et al. 2014). However, phylogenetic analysis within the Perissodactyla confirms the close relationship between horses and tapirs (Price and Bininda-Emonds 2009) suggesting that EIA spillover from horses to tapirs is plausible (Pedersen et al. 2007).

Horses spatially overlapping with the distributions of tapirs may not be new phenomena. It has been suggested that in 1519 Spaniards introduced horses into the Americas. This introduction apparently occurred in Mexico, later extending horse populations throughout the Americas (Fazio 1995). Our overall results suggest that without effective management, biodiversity reserves may not protect wildlife from disease spillover from domestic animals. We argue that infected horses should be removed from the natural reserve considering that (i) no vaccine is currently available for EIA, (ii) infected animals are potential reservoirs for their entire life, (iii) blood-sucking flies are present in the area, and (iv) there is limited understanding of the potential effects of EIA in tapirs.

AUTHOR´S CONTRIBUTION

MALL data collection, analysis, and document writing; RGA conception and document writing; NFJ document writing; GP conception and design and document writing; MG conception, data acquisition, and document writing; LEE document writing.

ACKNOWLEDGMENTS

FUNDESA (Fundación para el desarrollo de Guatemala) and PACUNAM (Fundación Patrimonio Cultural y Natural Maya) provided funding for the development of this study. Roan Mcnab supported the field work component. Authors thank Meggan E. Craft for her invaluable suggestions during the preparation of this manuscript.

LITERATURE CITED

Aldán EC, Torres IL, Andrade DMG, Sarabia DO, Quintero MT. 2006. Parasites of the Central American tapir Tapirus bairdii (Perissodactyla: Tapiridae) in Chiapas, Mexico. Rev. Biol. Trop. 54(2):445-450. [ Links ]

Coggins L, Norcross NL, Nusbaum SR. 1972. Diagnosis of equine infectious anemia by immunodiffusion test. Am. J. Vet. Res. 33(1):11-18. [ Links ]

[CONAP] Consejo Nacional de Áreas Protegidas. c2001. Plan Maestro de la Reserva de la Biosfera Maya. Guatemala: [last accessed: 24 Feb 2016]. http://theredddesk.org/sites/default/files/plan_maestro_rbm_2001-2006.pdf . [ Links ]

Craft ME, Volz E, Packer C, Meyers LA. 2009. Distinguishing epidemic waves from disease spillover in a wildlife population. Proc. R Soc. Lond. B Biol. Sci. 276:1777-1785. doi: 10.1098/rspb.2008.1636. [ Links ]

Fazio PM. 1995. The fight to save a memory: Creation of the Pryor Mountain Wild Horse Range (1968) and evolving federal wild horse protection through 1971. [Tesis]. [Texas]: Texas A&M University. [ Links ]

Furtado MM, Jácomo AT, Kashivakura CK, Tôrres NM, Marvulo MF, Ragozo AM, de Souza SL, Neto JS, Vasconcellos SA, Morais ZM, Cortez A, Richtzenhain LJ, Silva JC, Silveira L. 2010. Serologic survey for selected infectious diseases in free-ranging Brazilian tapirs (Tapirus terrestris) in the Cerrado of Central Brazil. J. Zoo Wildl. Med. 41(1):133-136. doi: 10.1638/2007-0087.1. [ Links ]

García M, Castillo F, García L, Leonardo R. c2009. El Tapir Centroamericano (Tapirus bairdii) como herramienta para el fortalecimiento del Sistema Guatemalteco de Áreas Protegidas. [last accessed: 24 Feb 2016]. http://digi.usac.edu.gt/bvirtual/informes/puirna/INF-2009-052.pdf . [ Links ]

García M, Jordan C, O’Farril G, Poot C, Meyer N, Estrada N, Leonardo R, Naranjo E, Simons Á, Herrera A, et al. c2017. Tapirus bairdii. The IUCN Red List of Threatened Species 2016 web site. [last accessed: 20 Nov 2017]. http://dx.doi.org/10.2305/IUCN.UK.2016-1.RLTS.T21471A45173340.en . [ Links ]

Issel CJ, Foil LD. 1984. Studies on equine infectious anemia virus transmission by insects. J. Am. Vet. Med. Assoc. 184(3):293-297. [ Links ]

Mangini PR, Medici EP, Fernandes Santos RC. 2012. Tapir health and conservation medicine. Integr. Zool. 7(4):331-345. doi: 10.1111/j.1749-4877.2012.00323.x. [ Links ]

May Júnior JA. 2011. Avaliação de Parâmetros Fisiológicos e Epidemiológicos da População de Anta-brasileira (Tapirus terrestris, Linnaeus, 1758) na Mata Atlântica do Parque Estadual Morro do Diabo, Pontal do Paranapanema, São Paulo [Tesis.] [Sao Pablo.]: Universidade de São Paulo USP. [ Links ]

Medici EP, Mangini PR, Fernandes-Santos RC. 2014. Health Assessment of Wild Lowland Tapir (Tapirus terrestris) Populations in the Atlantic Forest and Pantanal Biomes, Brazil (1996-2012). J. Wildl Dis. 50(4):817-828. doi: 10.7589/2014-02-029. [ Links ]

Monzón J, Schuster J. 2013. Mayab Yik’elil Kan: The Tropical Insects of the Ancient Kan Kingdom of Mesoamerica. FARES Foundation. Corporacion Litográfica S.A., Guatemala; I edition. [ Links ]

Pedersen AB, Jones KE, Nunn CL, Altizer S. 2007. Infectious diseases and extinction risk in wild mammals. Conserv. Biol. 21(5):1269-1279. doi: 10.1111/j.1523-1739.2007.00776.x. [ Links ]

Power AG, Mitchell CE. 2004. Pathogen spillover in disease epidemics. Am. Nat. 164(S5): S79- S89. doi: 10.1086/424610. [ Links ]

Price SA, Bininda-Emonds ORP. 2009. A comprehensive phylogeny of extant horses, rhinos and tapirs (Perissodactyla) through data combination. Zoosyst. Evol. 85(2):277-292. doi: 10.1002/zoos.200900005. [ Links ]

Vettorazzi M. Manrique RL. 2016. Classification of potential habitat of the Central American tapir (Tapirus bairdii Gill, 1865) for their conservation in Guatemala. Therya. 7(1):107-121. doi: 10.12933/therya-16-345. [ Links ]

CONFLICT OF INTEREST The authors declare that there is no conflict of interests.

Received: April 08, 2017; Accepted: January 02, 2018

* Corresponding author: malepelopez@gmail.com

Creative Commons License This is an open-access article distributed under the terms of the Creative Commons Attribution License