<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0120-0488</journal-id>
<journal-title><![CDATA[Revista Colombiana de Entomología]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. Colomb. Entomol.]]></abbrev-journal-title>
<issn>0120-0488</issn>
<publisher>
<publisher-name><![CDATA[Sociedad Colombiana de Entomología]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0120-04882003000200004</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Técnicas moleculares como herramientas en la epidemiología: Análisis de la dispersión y posibilidad de reinfestación por Rhodnius colombiensis(Hemiptera: Reduviidae)]]></article-title>
<article-title xml:lang="en"><![CDATA[Molecular techniques as tools in the epidemiology field: Analysis of dispersión and reinfestation possibilityby Rhodnius colombiensis (Hemiptera: Reduviidae)]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[VERGEL M]]></surname>
<given-names><![CDATA[CAROLINA]]></given-names>
</name>
<xref ref-type="aff" rid="A01"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[JARAMILLO H]]></surname>
<given-names><![CDATA[CARLOS]]></given-names>
</name>
<xref ref-type="aff" rid="A02"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[CASTRO]]></surname>
<given-names><![CDATA[LYDA]]></given-names>
</name>
<xref ref-type="aff" rid="A03"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[VALLEJO]]></surname>
<given-names><![CDATA[GUSTAVO]]></given-names>
</name>
<xref ref-type="aff" rid="A04"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[GUHL]]></surname>
<given-names><![CDATA[FELIPE]]></given-names>
</name>
<xref ref-type="aff" rid="A05"/>
</contrib>
</contrib-group>
<aff id="A01">
<institution><![CDATA[,Universidad de los Andes Departamento de Ciencias Biológicas Centro de Investigaciones en Microbiología y Parasitología Tropical - CIMPAT]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
</aff>
<aff id="A02">
<institution><![CDATA[,Universidad de los Andes Departamento de Ciencias Biológicas Centro de Investigaciones en Microbiología y Parasitología Tropical - CIMPAT]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
</aff>
<aff id="A03">
<institution><![CDATA[,Universidad de los Andes Departamento de Ciencias Biológicas Centro de Investigaciones en Microbiología y Parasitología Tropical - CIMPAT]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
</aff>
<aff id="A04">
<institution><![CDATA[,Universidad del Tolima Departamento de Ciencias Básicas Laboratorio de Investigaciones en Parasitología Tropical]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
</aff>
<aff id="A05">
<institution><![CDATA[,Universidad de los Andes Departamento de Ciencias Biológicas Centro de Investigaciones en Microbiología y Parasitología Tropical - CIMPAT]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2003</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2003</year>
</pub-date>
<volume>29</volume>
<numero>2</numero>
<fpage>137</fpage>
<lpage>142</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_arttext&amp;pid=S0120-04882003000200004&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_abstract&amp;pid=S0120-04882003000200004&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.co/scielo.php?script=sci_pdf&amp;pid=S0120-04882003000200004&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Se analizaron molecularmente insectos del género Rhodnius, pertenecientes al habitat doméstico y silvestre para determinar la posibilidad de reinfestación de las viviendas por parte de la población silvestre y así orientar apropiadamente los programas de control y vigilancia epidemiológica. Se generaron dendrogramas utilizando programas de cómputo, los cuales, junto con los valores calculados de Fst (0,2421) y Nm (1,05), sugieren que no existe flujo genético entre las dos poblaciones, es decir, que se trata de dos poblaciones aisladas. Sin embargo, se encontraron 4 individuos que fueron colectados dentro de las casas pero se caracterizaron como pertenecientes a la población silvestre mediante rDNA-PCR y adicionalmente se agruparon en el cluster de la población silvestre; esto indicaría un posible movimiento de individuos silvestres hacia áreas domiciliares, por lo tanto se recomienda monitorear periódicamente las viviendas para evitar una posible reinfestación.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Insects of the genus Rhodnius belonging to the domestic and silvatic habitat were analyzed by molecular techniques to determine the reinfestation possibility of the human dwellings to guide the control programs and epidemiological survellaince. Dendrographs, Fst (0.2421) and Nm (1.05) were generated using informatic packages. These results suggest that genetic flow between the two populations is nuil, that is to say, they are isolated. However, 4 individuáis captured in the houses, were characterized as belonging to the wild population when rDNA was amplified, and also this individuáis grouped inside of the wild population cluster, this also suggest a possible migration of wild individuáis to domiciliated áreas, therefore it is recommended a períodic monitoring of human dwellings to prevent possible reinfestations.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[rDNA-PCR]]></kwd>
<kwd lng="es"><![CDATA[RAPD]]></kwd>
<kwd lng="es"><![CDATA[Flujo genético]]></kwd>
<kwd lng="es"><![CDATA[Programas de control]]></kwd>
<kwd lng="es"><![CDATA[Enfermedad de Chagas]]></kwd>
<kwd lng="es"><![CDATA[Fst]]></kwd>
<kwd lng="es"><![CDATA[Tasa de migración]]></kwd>
<kwd lng="en"><![CDATA[rDNA-PCR]]></kwd>
<kwd lng="en"><![CDATA[RAPD]]></kwd>
<kwd lng="en"><![CDATA[Qenetic flow]]></kwd>
<kwd lng="en"><![CDATA[Control programs]]></kwd>
<kwd lng="en"><![CDATA[Chagas disease]]></kwd>
<kwd lng="en"><![CDATA[Fst]]></kwd>
<kwd lng="en"><![CDATA[Migration rate]]></kwd>
</kwd-group>
</article-meta>
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<page-range>323-335</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
